Artificial breeding method for glyptosternon maculatum
It is a technology of artificial breeding and application in fish farming, application, climate change adaptation, etc. It can solve problems such as non-compliance with energy saving and emission reduction, and achieve the effects of high quality and safety, high density and high resource utilization.
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Embodiment 1
[0047] A kind of artificial breeding method of black-spotted catfish , including the following steps:
[0048] A. Selection of broodstock
[0049] After soaking in salt water, the wild black-spotted broodstock are identified as males and females, and the females and males are collected;
[0050] B. Artificial induced labor
[0051] The dosage of the pharmaceutical composition for inducing labor in female fish is: luteinizing hormone releasing hormone 5 μg / kg body weight + 1 carp pituitary gland / kg body weight; 24 hours after injection, lightly press the abdomen of female fish, if no free eggs flow out, then inject The second injection of the same dose of oxytocin;
[0052] C. Artificial insemination
[0053] Fertilization by dry method: take out the testis of the male fish, grind it, wash it with normal saline, put it into a container containing fish eggs, mix the sperm and eggs, put them into the hatching tray, and hatch them in the hatching tank;
[0054] D. seedling cu...
Embodiment 2
[0057] This embodiment is on the basis of embodiment 1:
[0058] In step A, soak for 20 minutes in saline with a mass fraction of 1%.
Embodiment 3
[0060] This embodiment is on the basis of embodiment 1:
[0061] In the step A, soak for 40 minutes in saline with a mass fraction of 1%.
[0062] Described A step, the body weight of collecting female fish is 0.065kg, and the body weight of male fish is 0.38kg.
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