Method for regulating and controlling peltate yam rhizome triploid plants to root

Abstract

The invention discloses a method for regulating and controlling peltate yam rhizome triploid plants to root. The method is characterized by comprising the specific steps of selecting peltate yam rhizome triploid regeneration buds which grow healthily, and cutting the regeneration buds into stems with buds, which are 2-3cm long, on a clean bench; inoculating the stems into a culture medium, wherein the culture medium comprises a 1 / 2MS, 0.3mg / L of NAA (Naphthalene Acetic Acid), 0.24-0.96mg / L of ecdysterone, 30000mg / L of sucrose and 7500mg / L of agar powder, and the pH value of the culture medium is 5.8-6.0; and culturing under aseptic conditions, wherein the culture conditions are as follows: the temperature is 22-24 DEG C, the stems are cultured at a bright place for 16 hours and at a dark place for 8 hours, the relative humidity is 80%-90% and the illumination intensity is 2000-3000Lx. The method has a remarkable facilitation effect on the rooting of the peltate yam rhizome triploid plants; and particularly, the rooting starting time can be shortened, the average root length and the average rooting quantity are remarkably increased, and the transplanting survival rate is also remarkably improved.

Description

technical field [0001] The invention belongs to the technical field of rooting methods for plant triploid plants, and in particular relates to a method for regulating the rooting of triploid plants of Dioscorea scutellalis. Background technique [0002] Dioscorea shield leaf ( Dioscorea Zingiberensis C.H.Wright) is a perennial herb of the genus Dioscorea in the Dioscoreaceae family, commonly known as turmeric. It is used as medicine with dried rhizomes. About 10% of the rhizomes are dioscin (the highest content is 16.5%) (Qin Songyun, Ding Jichun, Shu Shu, Yu Zaibai, Li Quansen. Dioscorea shield leaf resource status and protection countermeasures in China. Resource Development and Market, 2004, 20, 263-265.), is the plant with the highest saponin content in the world. Diosgenin has the functions of lowering blood fat, anti-aging, anti-tumor, treating osteoporosis, protecting the liver, anti-diabetes and anti-inflammation. [0003] The dosage effect of polyploid gene can ma...

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Problems solved by technology

[0006] Through the summary of the research reports on EDS in the past 50 years, it is found that the relevant research mainly focuses on the isolation and identification, chemical structure, effect on animals and its mechanism, and the distribution, accumulation and determination methods in different plants. There are also many, but the effect on the plant itself has not been reported

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