Telecentric optical structure-based transmission-type digital holographic microscopic imaging device

Abstract

The invention discloses a telecentric optical structure-based transmission-type digital holographic microscopic imaging device. By a telecentric optical structure, two beams of parallel light, namely object light and reference light intervene on an imaging plane of a camera to form an interference pattern, so that aberration in a conventional digital holographic microscopic imaging device can be avoided; the accuracy of a system is greatly improved; other complicated physical or aberration compensation calculation processes are not needed; the imaging speed is improved; and the late calculating and processing complexity is lowered.

Description

technical field [0001] The invention belongs to optical measurement and imaging technology, in particular to a transmission digital holographic microscopic imaging device based on a telecentric optical structure. Background technique [0002] As a new type of coherent measurement and imaging technology, digital holography has the biggest advantage of being able to simultaneously and independently obtain quantitative amplitude information and phase information of objects. Phase information is particularly important when performing quantitative detection of almost transparent objects such as biological cells. However, when using the traditional digital holographic microscope system for quantitative phase measurement, in order to obtain the phase image of the object accurately, the phase distortion in the reconstructed image must be corrected first, which requires knowing the various parameters in the experiment, such as Record the distance, the included angle of the object pa...

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Problems solved by technology

However, the two-step exposure method needs to record two holograms, which requires high system stability

The M.K.Kim research group in the United States proposed a method of physical compensation ([4] M.K.Kim. "Applications of Digital Holography in Biomedical Microscopy". J.Opt.Soc.Korea.2010,14(2):77~89), this method does not Instead of using a tube lens in the light path, another identical microscope objective lens was added to the reference light path in an attempt to make the curvature of the spherical reference light the same as that of the spherical object light, thereby eliminating the secondary phase distortion in the experimental recording, However, the position of the second microscope objective lens added by this method is difficult to determine and adjust, and a slight position shift will cause phase distortion that cannot be completely eliminated

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