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Stable adenosine deaminase reagent high in anti-interference capability and detection method

A technology of adenosine deaminase and detection method, which is applied in the directions of biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem that batch samples cannot be used for determination, etc., and achieves enhanced anti-interference ability, accuracy and stability. Good, performance-improving effect

Inactive Publication Date: 2016-01-13
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is simple to operate and easy to popularize, but it cannot be used for the determination of batch samples

Method used

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  • Stable adenosine deaminase reagent high in anti-interference capability and detection method
  • Stable adenosine deaminase reagent high in anti-interference capability and detection method
  • Stable adenosine deaminase reagent high in anti-interference capability and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Adenosine deaminase detection reagent, including reagent R1 and reagent R2:

[0048] 1) The composition of its R1 is:

[0049] buffer ·········································································· 100mmol / L

[0050] 4-AA ··············································································· 4mmol / L

[0051] PNP ················································································ 0.3U / mL

[0052] XOD ················································································ 0.4U / mL

[0053] PODS ·················································································· 0.1U / mL

[0054] BSA ····················································································· 1g / L,

[0055] sucrose ···················································································· 5g / L,

[0056] Trehalose ················································································· 2g / L,

[0057] Peroxidase ...

Embodiment 2

[0072]Interference test: take fresh mixed serum, divide it into 2 equal parts, then divide each equal part into 5 equal parts, add different interfering substances, so that the concentration in the serum reaches the requirements in Table 2. Then use the reagent obtained in Example 1 to compare with the common and recognized adenosine deaminase (ADA) reagent in the market to measure the ADA content in serum. The results of the control group and the results of each group after adding different interfering substances are shown in the table 2. Relative deviation (%) = (measuring mean value of interference samples - measuring mean value of control samples) / measured mean value of control samples × 100%.

[0073] Depend on Figure four It can be seen that the reagent of Example 1 does not significantly interfere with the test results when ascorbic acid≤1704μmol / L, bilirubin≤684μmol / L, hemoglobin≤10g / L, and triglyceride≤22.6mmol / L. However, the reagents of the control group were sig...

Embodiment 3

[0075] Correlation experiment: using the formula in Example 1 to prepare reagents, and conducting a control test with the ADA kit of a company approved by the State Food and Drug Administration, which is common in the market, and testing 20 clinical serum samples at the same time, the test results are as follows: Figure five shown. And obtained the correlation curve of the two reagents (such as figure 1 Shown), the test results show that the correlation coefficient of the two kits is 0.9992, indicating that there is a great correlation between the two.

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Abstract

The invention relates to the technical field of adenosine deaminase detection, in particular to an adenosine deaminase detection reagent. A reagent R1 comprises a buffer solution, 4-aminoantipyrine, BSA, cane sugar, trehalose, peroxidase, ascorbic acid oxidase, bilirubin oxidase and preservatives; a reagent R2 comprises a buffer solution, adenosine, EHSPT, BSA, cane sugar and preservatives. The adenosine deaminase detection reagent has the advantages that the phosphate buffer solution is adopted, the stabilizer BSA, the cane sugar and the trehalose are added, and accordingly, stability of the reagent is improved greatly; determination performance is improved remarkably, and stability and anti-interference capability of the reagent are enhanced.

Description

technical field [0001] The invention relates to the technical field of adenosine detection, in particular to an adenosine deaminase detection reagent and a detection method using the detection reagent. Background technique [0002] Adenosine deaminase (ADA) is a purine nucleotide catabolic enzyme that has an important relationship with the immune activity of the body's cells. In the late 1950s, the determination of ADA activity was introduced into the field of enzymology, and it has been used in various diseases diagnosis. ADA can specifically catalyze the irreversible deamination reaction of adenosine. The reduction of ADA activity will affect the synthesis of nucleosides, and reduce the ability of lymphoid progenitor cells to transform into lymphoblasts and plasma cells, resulting in the reduction of immune active cells and functional decline. ADA activity in body fluids has become an important clinical indicator for the diagnosis and differential diagnosis, curative e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/34C12Q1/28C12Q1/26
Inventor 甘宜梧史秀明谢清华谭柏清王绮赵新
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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