Conventional low-temperature preservation method of volvariella volvacea strain

Abstract

The invention relates to a conventional low-temperature preservation method of a volvariella volvacea strain. The preservation method comprises the following steps: (1) transferring the volvariella volvacea strain onto a PDA (potato dextrose agar) plate, performing static culturing in an incubator at 30-32 DEG C, then transferring the strain to a PDA test tube slant after mycelia permeate the plate, performing static culturing at 30-32 DEG C for 3-7d; (2) preparing a mannitol solution which is 5-20% in mass fraction, and then filtering and sterilizing; and (3) transferring the mannitol solution in the step (2) to the test tube slant which is full of the mycelia in the step (1) by virtue of a sterile injector, and preserving the test tube slant at conventional low temperature. The preservation method disclosed by the invention can prolong a preservation duration of the volvariella volvacea mycelia in a low-temperature environment of 3-6 DEG C; the preservation method has the advantages of simple and convenient operation, low cost, long preservation duration of the strain, rapid recovery and growth, good stability and the like; and the preservation method is especially suitable for small and medium-sized enterprises and farmers planting the volvariella volvacea.

Description

technical field [0001] The invention belongs to the field of preservation of edible fungus strains, in particular to a conventional low-temperature preservation method of straw mushroom strains. Background technique [0002] Straw mushroom (Volvariella volvacea (Bullex Fr.) Sing), also known as straw mushroom, orchid mushroom, Chinese mushroom, is an important saprophytic edible fungus, which originated in tropical and subtropical high-temperature and rainy areas in my country. Straw mushroom meat is tender and delicious, rich in protein, amino acids, fatty acids, polysaccharides, vitamins, etc. Among them, the protein content is 27.24%-30.55%, and the content of 8 essential amino acids for the human body is as high as 41.54%. Polysaccharides, unsaturated fatty acids, vitamins, minerals, etc. in straw mushrooms have important functions of delaying aging, enhancing immunity, preventing tumors, and lowering cholesterol. [0003] Straw mushroom is a high-temperature mushroom s...

AI Technical Summary

Problems solved by technology

The simple preservation method refers to placing the mycelium of straw mushrooms covered with the slope of the test tube in an incubator at 15-20°C, and transferring the tube once every 2-3 months. The operation is cumbersome and it is easy to transfer multiple times. Cause the degradation of strains and the contamination of miscellaneous bacteria; at the same time, the required 15-20°C culture conditions are difficult to control, not as convenient as the low temperature conditions of 3-6°C, and the low-temperature preservation of strains can be realized by using household refrigerators

Freeze-drying preservation method and liquid nitrogen cryopreservation method, although the preservation time of bacteria is longer, but the equipment is expensive, and the cost of dry ice and liquid nitrogen is high, which is only suitable for large-scale culture preservation centers or laboratories

For general straw mushroom cultivation enterprises, especially for farmers, these methods are difficult to implement in actual production

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