Preparation method for low-molecular heparin originated from new species

Abstract

The invention relates to a preparation method for low-molecular heparin originated from a new species. The preparation method comprises the following steps: (1) fully dissolving heparin sodium prepared from raw materials bovine lungs, ox intestines and / or goat intestines in water, and adding a benzethonium chloride solution to prepare heparin benzethonium chloride salt; (2) dissolving the heparin benzethonium chloride salt in dichloromethane, adding benzyl chloride, stirring the mixture to react, reducing the temperature and adding a sodium acetate methanol solution to prepare heparin benzyl ester; (3) dissolving the heparin benzyl ester in water, adding sodium hydroxide, reducing the temperature after reaction, adjusting the pH to neutral, adding sodium chloride, and then adding alcohol to prepare an enoxaparin sodium crude product; and (4) dissolving the enoxaparin sodium coarse product in water, and purifying and drying the mixture to obtain enoxaparin sodium. According to the preparation method provided by the invention, the condition that the low-molecular heparin can be obtained by heparin sodium prepared from bovine lungs, ox intestines and goat intestines is found for the first time, and the prepared low-molecular heparin is lower in molecular weight compared with that of existing low-molecular heparin, so that the biological activity is higher and the low-molecular heparin has a wide market application prospect.

Description

technical field [0001] The invention relates to a method for preparing low-molecular-weight heparin from a new species, in particular to a method for preparing low-molecular-weight heparin from bovine (bovine lung, bovine intestine) and sheep (sheep intestine) heparin, and belongs to the technical field of preparation of raw materials Background technique [0002] Heparin and low-molecular-weight heparin belong to the anticoagulant glycosaminoglycan clinical drug. Low-molecular-weight heparin is a new type of antithrombotic drug prepared from heparin by physical methods, chemical methods or enzymatic hydrolysis. Compared with heparin, low-molecular-weight heparin has higher biological activity, longer half-life and fewer side effects. Low-molecular-weight heparin drugs have become the first choice for antithrombotic and anticoagulant drugs. Heparin can be extracted from mucous membrane tissues such as pig small intestine, bovine lung, bovine intestine, and sheep small intest...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem that the source species of the existing low-molecular-weight heparins is single and the molecular weight of the obtained low-molecular-weight heparin is still relatively large, the present invention provides a method for preparing low-molecular-weight heparin from a new species

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