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Kit for activating esophagus cancer specific immunity response

An immune response and kit technology, used in the field of kits for activating esophageal cancer-specific immune responses

Inactive Publication Date: 2016-01-06
SHANGHAI LONGYAO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, DC1 cells highly expressing IL12 loaded with tumor-specific antigen peptides can provide a strategy for enhancing the efficacy of antigen peptide-based tumor therapy, but there is no corresponding, mature and suitable for the activation of esophageal cancer in the prior art. Kits for Patient-Specific Immune Responses

Method used

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  • Kit for activating esophagus cancer specific immunity response
  • Kit for activating esophagus cancer specific immunity response
  • Kit for activating esophagus cancer specific immunity response

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 (cell culture supernatant is T cell culture supernatant)

[0034] When the kit of the present invention is used to activate the specific immune response of esophageal cancer patients, the preparation of type 1 polarized dendritic cells (type1 polarized dendritic cells, DC1) derived from adult peripheral blood is firstly performed. Peripheral blood mononuclear cells were isolated according to the method described by Jonuleit et al. (GeneTher. 200310(3)). First, peripheral blood mononuclear cells were separated by Ficoll-Hypaque density gradient centrifugation, and plasma was collected for subsequent culture. By density 3.0×10 6 / ml, the cells were inoculated into T75 culture flasks, and 10% plasma was added to the GGT551 medium, placed at 37.0°C, saturated humidity, 5% CO 2 cultivated in the environment. After culturing for 3 hours, the suspended lymphocytes were washed away, and DMEM / F12 medium containing GM-CSF and IL-4 1000 IU / ml and 10% plasma was add...

Embodiment 2

[0039] Embodiment two (cell culture supernatant is NK cell and K562 cell co-culture supernatant)

[0040] Peripheral blood mononuclear cells were isolated according to the method described by Jonuleit et al. (GeneTher. 200310(3)). First, peripheral blood mononuclear cells were separated by Ficoll-Hypaque density gradient centrifugation, and plasma was collected for subsequent culture. IL-2 (2000U / ml) and IL15 (2ng / ml) were added. Doubling the medium every two to three days, and supplementing the factors in equal amounts. Collected on the twelfth day. After the induction is completed, NK cells are treated by 1-3×10 6 / ml was inoculated into medium RPMI1640, IFNa (1000UI / ml) was added and 0.5-1.5×10 5 Inoculate K562 cells (can be replaced with K562-NK cells) at a density of / ml for mixed culture, collect the medium supernatant during 24-48 hours of activation, filter and freeze at -80 degrees or directly use it for the preparation of DC1 cells. Peripheral blood mononuclear ...

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Abstract

The invention provides a kit for activating esophagus cancer specific immunity response. The kit comprises an RPMI-1640 culture medium, a cell culture supernatant, a GM-CSF, an IL-4, an INF gamma and an esophagus cancer specific antigenic peptide combination. The cell culture supernatant is an NK cell and K562 cell co-culture supernatant or a T cell culture supernatant. The esophagus cancer specific antigenic peptide combination includes MAGE-A3-A2 antigenic peptides, MAGE-A4-A2 / 3 antigenic peptides, NY-ESO-1-A2 antigenic peptides, CEA-A2 antigenic peptides, CEA-A3 antigenic peptides, hTERT-A2 antigenic peptides, hTERT-A3 antigenic peptides, Survivin-A2 antigenic peptides, Survivin-A3 antigenic peptides, COX-2-A2 / 3 antigenic peptides and MTA1-A2 antigenic peptides. The kit can efficiently activate esophagus cancer specific immunity response in an active inducing / passive inducing combined mode.

Description

technical field [0001] The invention relates to a kit for enhancing anti-cancer immunity, in particular to a kit for activating esophageal cancer specific immune response. Background technique [0002] Esophageal cancer is a common digestive tract tumor, and about 300,000 people worldwide die from esophageal cancer every year. Its morbidity and mortality vary widely from country to country. my country is one of the areas with a high incidence of esophageal cancer in the world, with an average of 150,000 deaths per year. There are more men than women, and the age of onset is more than 40 years old. Patients suffering from esophageal cancer often suffer from great pain. Generally, they have progressive dysphagia. First, it is difficult to swallow dry food, then semi-liquid food, and finally water and saliva cannot be swallowed. [0003] Since entering the 21st century, great advances in molecular biology and immunology have provided new strategies for clinical tumor treatme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0784C12N5/0783A61K39/00A61P35/00
Inventor 李伟叶圣勤汪鑫瞿苏
Owner SHANGHAI LONGYAO BIOTECH CO LTD
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