Kit for activating breast cancer specific immunity response

An immune response and kit technology, applied in the field of kits for activating breast cancer-specific immune responses

Inactive Publication Date: 2016-01-06
SHANGHAI LONGYAO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, DC1 cells highly expressing IL12 loaded with tumor-specific antigen peptides can provide a strategy for enhancing the efficacy of antigen peptide-based tumor therapy, but there is no corresponding, mature and suitable for activating breast cancer in the prior art. Kits for Patient-Specific Immune Responses

Method used

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  • Kit for activating breast cancer specific immunity response
  • Kit for activating breast cancer specific immunity response
  • Kit for activating breast cancer specific immunity response

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 (cell culture supernatant is T cell culture supernatant)

[0036] When the kit of the present invention is used to activate the specific immune response of breast cancer patients, firstly, the preparation of type 1 polarized dendritic cells (type1 polarized dendritic cells, DC1) derived from adult peripheral blood is carried out. Peripheral blood mononuclear cells were isolated according to the method described by Jonuleit et al. (GeneTher. 200310(3)). First, peripheral blood mononuclear cells were separated by Ficoll-Hypaque density gradient centrifugation, and plasma was collected for subsequent culture. By density 3.0×10 6 / ml, the cells were inoculated into T75 culture flasks, and 10% plasma was added to the GGT551 medium, placed at 37.0°C, saturated humidity, 5% CO 2 cultivated in the environment. After culturing for 3 hours, the suspended lymphocytes were washed away, and DMEM / F12 medium containing GM-CSF and IL-4 1000 IU / ml and 10% plasma was adde...

Embodiment 2

[0041] Embodiment two (cell culture supernatant is NK cell and K562 cell co-culture supernatant)

[0042] Peripheral blood mononuclear cells were isolated according to the method described by Jonuleit et al. (GeneTher. 200310(3)). First, peripheral blood mononuclear cells were separated by Ficoll-Hypaque density gradient centrifugation, and plasma was collected for subsequent culture. IL-2 (2000U / ml) and IL15 (2ng / ml) were added. Doubling the medium every two to three days, and supplementing the factors in equal amounts. Collected on the twelfth day. After the induction is completed, NK cells are treated by 1-3×10 6 / ml was inoculated into medium RPMI1640, IFNa (1000UI / ml) was added and 0.5-1.5×10 5 Inoculate K562 cells (can be replaced with K562-NK cells) at a density of / ml for mixed culture, collect the medium supernatant during 24-48 hours of activation, filter and freeze at -80 degrees or directly use it for the preparation of DC1 cells. Peripheral blood mononuclear ...

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Abstract

The invention provides a kit for activating breast cancer specific immunity response. The kit comprises an RPMI-1640 culture medium, a cell culture supernatant, a GM-CSF, an IL-4, an INF gamma and a breast cancer specific antigenic peptide combination. The cell culture supernatant is an NK cell and K562 cell co-culture supernatant or a T cell culture supernatant. The breast cancer specific antigenic peptide combination includes MAGE-A1-A2 antigenic peptides, MAGE-A1-A3 antigenic peptides, NY-ESO-1-A2 antigenic peptides, PLAC1-A2 antigenic peptides, PIWIL2-A2 antigenic peptides, CEA-A2 antigenic peptides, CEA-A3 antigenic peptides, Mamaglobin-A-A3 antigenic peptides, NY-BR-1-A2 antigenic peptides, HER2-A2 antigenic peptides, HER2-A3 antigenic peptides and MTA1-A2. The kit can efficiently activate breast cancer specific immunity response in an active inducing / passive inducing combined mode.

Description

technical field [0001] The invention relates to a kit for enhancing anti-cancer immunity, in particular to a kit for activating breast cancer specific immune response. Background technique [0002] The female mammary gland is composed of skin, fibrous tissue, mammary glands and fat. Breast cancer is a malignant tumor that occurs in the mammary gland epithelial tissue. 99% of breast cancers occur in women and only 1% in men. [0003] The mammary gland is not an important organ to maintain human life activities, and breast cancer in situ is not fatal; but because breast cancer cells lose the characteristics of normal cells, the connections between cells are loose and easy to fall off. Once the cancer cells fall off, the free cancer cells can spread throughout the body with the blood or lymph fluid, forming metastasis, which is life-threatening. At present, breast cancer has become a common tumor that threatens women's physical and mental health. [0004] The global incidenc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0784C12N5/0783A61K39/00A61P35/00
Inventor 李伟叶圣勤汪鑫瞿苏
Owner SHANGHAI LONGYAO BIOTECH CO LTD
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