Kit for activating lung cancer specific immunity response

An immune response and kit technology, applied in the field of kits for enhancing anti-cancer immunity

Inactive Publication Date: 2016-01-06
SHANGHAI LONGYAO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, DC1 cells highly expressing IL12 loaded with tumor-specific antigen peptides can provide a strategy for enhancing the efficacy of antigen peptide-based tumor therapy, but there is no corresponding, mature and suitable for activation of lung cancer patients in the prior art. Kits for specific immune responses

Method used

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  • Kit for activating lung cancer specific immunity response
  • Kit for activating lung cancer specific immunity response
  • Kit for activating lung cancer specific immunity response

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 (cell culture supernatant is T cell culture supernatant)

[0034] When the kit of the present invention is used to activate the specific immune response of lung cancer patients, the preparation of type 1 polarized dendritic cells (type1 polarized dendritic cells, DC1) derived from adult peripheral blood is firstly performed. Peripheral blood mononuclear cells were isolated according to the method described by Jonuleit et al. (GeneTher. 200310(3)). First, peripheral blood mononuclear cells were separated by Ficoll-Hypaque density gradient centrifugation, and plasma was collected for subsequent culture. By density 3.0×10 6 / ml, the cells were inoculated into T75 culture flasks, and 10% plasma was added to the GGT551 medium, placed at 37.0°C, saturated humidity, 5% CO 2 cultivated in the environment. After culturing for 3 hours, the suspended lymphocytes were washed away, and DMEM / F12 medium containing GM-CSF and IL-4 1000 IU / ml and 10% plasma was added. A...

Embodiment 2

[0039] Embodiment two (cell culture supernatant is NK cell and K562 cell co-culture supernatant)

[0040] Peripheral blood mononuclear cells were isolated according to the method described by Jonuleit et al. (GeneTher. 200310(3)). First, peripheral blood mononuclear cells were separated by Ficoll-Hypaque density gradient centrifugation, and plasma was collected for subsequent culture. IL-2 (2000U / ml) and IL15 (2ng / ml) were added. Doubling the medium every two to three days, and supplementing the factors in equal amounts. Collected on the twelfth day. After the induction is completed, NK cells are treated by 1-3×10 6 / ml was inoculated into medium RPMI1640, IFNa (1000UI / ml) was added and 0.5-1.5×10 5 Inoculate K562 cells (can be replaced with K562-NK cells) at a density of / ml for mixed culture, collect the medium supernatant during 24-48 hours of activation, filter and freeze at -80 degrees or directly use it for the preparation of DC1 cells. Peripheral blood mononuclear ...

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Abstract

The invention provides a kit for activating lung cancer specific immunity response. The kit comprises an RPMI-1640 culture medium, a cell culture supernatant, a GM-CSF, an IL-4, an INF gamma and a lung cancer specific antigenic peptide combination. The cell culture supernatant is an NK cell and K562 cell co-culture supernatant or a T cell culture supernatant. The lung cancer specific antigenic peptide combination includes MAGE-A1-A2 antigenic peptides, MAGE-A1-A3 antigenic peptides, MAGE-A3-A2 antigenic peptides, MAGE-A4-A2 / 3 antigenic peptides, CEA-A2 antigenic peptides, CEA-A3 antigenic peptides, hTERT-A2 antigenic peptides, hTERT-A3 antigenic peptides, Survivin-A2 antigenic peptides and Survivin-A3 antigenic peptides. The kit can efficiently activate lung cancer specific immunity response in an active inducing / passive inducing combined mode.

Description

technical field [0001] The invention relates to a kit for enhancing anti-cancer immunity, in particular to a kit for activating lung cancer specific immune response. Background technique [0002] Lung cancer is one of the malignant tumors with the fastest increasing morbidity and mortality and the greatest threat to the health and life of the population. In the past 50 years, many countries have reported that the morbidity and mortality of lung cancer have increased significantly. The incidence and mortality of lung cancer in men rank first among all malignant tumors, and the incidence and mortality of lung cancer occupy the second place in women. [0003] Since entering the 21st century, great advances in molecular biology and immunology have provided new strategies for clinical tumor treatment. A large number of tumor cell-specific antigens have been identified by means of molecular biology, and their recognition sites by immune cells have been analyzed by means of bioinf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0784C12N5/0783A61K39/00A61P35/00
Inventor 李伟叶圣勤汪鑫瞿苏
Owner SHANGHAI LONGYAO BIOTECH CO LTD
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