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Kit for activating head-neck cancer specific immunity response

A technology of immune response and cancer-specific antigen, applied in the field of kits for activating head and neck cancer-specific immune response

Inactive Publication Date: 2016-01-06
SHANGHAI LONGYAO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, DC1 cells highly expressing IL12 loaded with tumor-specific antigen peptides can provide a strategy for enhancing the efficacy of antigen peptide-based tumor therapy, but there is no corresponding, mature and suitable for activation of head and neck tumors in the prior art A kit for patient-specific immune responses to local cancer

Method used

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  • Kit for activating head-neck cancer specific immunity response
  • Kit for activating head-neck cancer specific immunity response
  • Kit for activating head-neck cancer specific immunity response

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 (cell culture supernatant is T cell culture supernatant)

[0034] When the kit of the present invention is used to activate the specific immune response of the head and neck cancer patient, the preparation of type 1 polarized dendritic cells (type1 polarized dendritic cells, DC1) derived from adult peripheral blood is firstly performed. Peripheral blood mononuclear cells were isolated according to the method described by Jonuleit et al. (GeneTher. 200310(3)). First, peripheral blood mononuclear cells were separated by Ficoll-Hypaque density gradient centrifugation, and plasma was collected for subsequent culture. By density 3.0×10 6 / ml, the cells were inoculated into T75 culture flasks, and 10% plasma was added to the GGT551 medium, placed at 37.0°C, saturated humidity, 5% CO 2 cultivated in the environment. After culturing for 3 hours, the suspended lymphocytes were washed away, and DMEM / F12 medium containing GM-CSF and IL-4 1000 IU / ml and 10% plasma w...

Embodiment 2

[0039] Embodiment two (cell culture supernatant is NK cell and K562 cell co-culture supernatant)

[0040] Peripheral blood mononuclear cells were isolated according to the method described by Jonuleit et al. (GeneTher. 200310(3)). First, peripheral blood mononuclear cells were separated by Ficoll-Hypaque density gradient centrifugation, and plasma was collected for subsequent culture. IL-2 (2000U / ml) and IL15 (2ng / ml) were added. Doubling the medium every two to three days, and supplementing the factors in equal amounts. Collected on the twelfth day. After the induction is completed, NK cells are treated by 1-3×10 6 / ml was inoculated into medium RPMI1640, IFNa (1000UI / ml) was added and 0.5-1.5×10 5 Inoculate K562 cells (can be replaced with K562-NK cells) at a density of / ml for mixed culture, collect the medium supernatant during 24-48 hours of activation, filter and freeze at -80 degrees or directly use it for the preparation of DC1 cells. Peripheral blood mononuclear ...

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Abstract

The invention provides a kit for activating head-neck cancer specific immunity response. The kit comprises an RPMI-1640 culture medium, a cell culture supernatant, a GM-CSF, an IL-4, an INF gamma and a head-neck cancer specific antigenic peptide combination. The head-neck cancer specific antigenic peptide combination includes MAGE-A1-A2 antigenic peptides, MAGE-A1-A3 antigenic peptides, MAGE-A3-A2 antigenic peptides, MAGE-A4-A2 / 3 antigenic peptides, CEA-A2 antigenic peptides, hTERT-A2 antigenic peptides, hTERT-A3 antigenic peptides, Survivin-A2 antigenic peptides and Survivin-A3 antigenic peptides. The kit can efficiently activate head-neck cancer specific immunity response in an active inducing / passive inducing combined mode.

Description

technical field [0001] The invention relates to a kit for enhancing anti-cancer immunity, in particular to a kit for activating head and neck cancer specific immune response. Background technique [0002] Head and neck tumors include tumors originating from any tissue or organ in the head and neck except the eye, brain, ear, thyroid, and esophagus, and should include neck tumors, ENT tumors, and oral and maxillofacial tumors. Neck tumors belong to general surgery in general hospitals, and the more common ones are thyroid tumors; common otolaryngology tumors include laryngeal cancer, paranasal sinus cancer, etc.; oral and maxillofacial tumors are common oral cancers, such as tongue cancer, Gum cancer, buccal cancer, etc. Therefore, the tumors that occur in the head and neck have many primary sites and pathological types, ranking first in systemic tumors. More than 90% of head and neck tumors are squamous cell carcinomas. The incidence of squamous cell carcinoma of the head...

Claims

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Application Information

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IPC IPC(8): C12N5/0784
Inventor 李伟叶圣勤汪鑫瞿苏
Owner SHANGHAI LONGYAO BIOTECH CO LTD
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