Multifunctional gold nanoflower drug compound carrier material and preparation method thereof

A technology of composite carrier and gold nanoflowers, which is applied in the direction of drug combination, wave energy or particle radiation treatment materials, pharmaceutical formulations, etc., can solve the problems of poor controllability of drug release and lack of universality, and achieve good near-infrared photothermal Controlled release, good stability and uniform particle size

Inactive Publication Date: 2016-01-06
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The above-mentioned multi-functional drug-loaded nanocomposite carrier, in order to achieve its targeting, often simultaneously surface-modifies monoclonal antibodies, aptamers, short peptides, sugars, small molecules, etc., and the drug is realized by changing the pH value and temperature of the surrounding environment. Selective release of drugs, but the currently prepared nano-gold composite drug carriers still have shortcomings such as lack of universality, poor controllability of drug release, and the stability of gold nanoparticles needs to be improved.

Method used

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  • Multifunctional gold nanoflower drug compound carrier material and preparation method thereof
  • Multifunctional gold nanoflower drug compound carrier material and preparation method thereof
  • Multifunctional gold nanoflower drug compound carrier material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation of multifunctional gold nanoflower drug composite carrier.

[0040] a. Prepare gold nano seeds by the Frens method:

[0041] Under stirring conditions, add 1% sodium citrate aqueous solution to the hot boiling 0.25mM tetrachloroauric acid aqueous solution, the molar ratio of sodium citrate to tetrachloroauric acid is 7:1, until the solution turns deep wine red After the color remains unchanged within 15 minutes, stop heating and stirring, and centrifuge after natural cooling to obtain precipitate spherical gold nanoparticles;

[0042] b. Preparation of gold nanoflowers:

[0043] 1) Disperse the gold nano seeds obtained in step a in a sodium citrate aqueous solution with a concentration of 1.75 mM to make the concentration of 5.18×10 -11 mol / L;

[0044] 2) Take the gold nanoseed solution and the ascorbic acid aqueous solution at a molar ratio of 200:1 to prepare a mixed solution, and adjust the pH of the mixed solution to 7, and then press the gold nanoseed to tetrach...

Embodiment 2

[0054] For the AuNFSiO prepared in Example 1 2 mSiO 2 Measurement test of near-infrared light-to-heat conversion effect of particles.

[0055] Use different powers of 808nm near-infrared laser to irradiate different concentrations of gold nanoflower composite particles, the power is 5w / cm respectively 2 ,7w / cm 2 And 9w / cm 2 , The sample concentration is 0ug / ml, 100ug / ml, 200ug / ml, 300ug / ml and 400ug / ml, take 2ml each, and irradiate each sample for 20 minutes. Figure 3 shows the temperature change value recorded in the experiment and plots the real-time curve of near-infrared photothermal conversion. It can be seen that the system temperature increases with the increase of particle concentration and irradiation power.

Embodiment 3

[0057] The biocompatibility test of the AuNFSiO2mSiO2 particle composite carrier material prepared in Example 1.

[0058] The CCK-8 method was used to detect normal liver HL-7702 cells, human liver tumor HepG2 cells and human tongue squamous cell carcinoma CAL27 cells. This method is easy to operate and has high sensitivity. Add AuNFsSiO2mSiO2 with concentrations of 0μg / ml, 6.25μg / ml, 12.50μg / ml, 25μg / ml, 50μg / ml and 100μg / ml to different cells and culture for 24 hours. The results are statistically analyzed by SPSS16.0 software . The test results are shown in Table 1, Table 2, and Table 3:

[0059] Table 1. CCK-8 test to detect AuNFSiO 2 mSiO 2 The effect of nanoparticles on the proliferation of Cal27 cells (n=6, %)

[0060]

[0061] Table 2. CCK-8 test to detect AuNFSiO 2 mSiO 2 Study on the effect of nanoparticles on the proliferation of Hep-G2 cells (n=6, )

[0062]

[0063] Table 3. CCK-8 test to detect AuNFSiO 2 mSiO 2 Nanoparticles study on the proliferation of HL7702 cell...

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Abstract

The invention relates to a multifunctional gold nanoflower drug compound carrier material and a preparation method thereof. A drug compound carrier is nano particles formed by taking gold nanoflower with a near infrared absorption function as a core, coating a layer of silicon dioxide on the surface of the gold nanoflower, and then coating a mesoporous silica layer on the silicon dioxide layer. The carrier material is characterized in that double-silicon shell coating is adopted, the outer mesoporous silica layer can be used for carrying multiple chemotherapeutics, photodynamics therapy drugs and the like and has universality, the inner silicon dioxide layer directly coats the gold nanoflower, avoids the curing of the gold nanoflower, enables material performance to be stable, the gold nanoflower with a near infrared photothermal conversion function can realize drug controllable release under near infrared light irradiation, and meanwhile a synergistic effect of chemotherapy, photodynamics therapy and near infrared photothermal treatment can be realized by carrying different drugs.

Description

Technical field [0001] The invention relates to a targeted drug delivery carrier material for disease treatment, in particular to a gold nanoparticle composite carrier material capable of achieving controlled release of drugs and a combined thermal and chemical treatment effect under near-infrared laser irradiation and a preparation method thereof . Background technique [0002] Targeted drug delivery and controlled release have always been a research hotspot in the medical field. With the development of nanomaterials technology, nano-scale mesoporous silica materials have become attractive drug carrier materials. Currently, surface adsorption and surface functional groups (such as propenyl) are commonly used for drug loading. In order to achieve good drug release, the drug can be achieved through temperature changes, environmental pH changes, and protease action around diseased tissues. freed. [0003] Nano-gold-mediated near-infrared hyperthermia is a new type of photothermal t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/51A61K47/02A61K47/04A61P35/00
Inventor 宋文植宫俊霞尹万忠黄珍珍张艳何鑫尹奕涵刘新苗强杨文胜
Owner JILIN UNIV
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