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Method for detecting biological membrane ageing state in sewage and wastewater processing system

A technology for processing systems and aging states, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as the inability to detect the aging state of biofilms, and achieve the effects of strong practicability, improved efficiency, and intuitive results

Active Publication Date: 2015-12-30
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem that the existing biofilm activity determination method cannot intuitively detect the biofilm aging state in the sewage and wastewater treatment system, the present invention provides a method for detecting the biofilm aging state in the sewage and wastewater treatment system

Method used

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  • Method for detecting biological membrane ageing state in sewage and wastewater processing system
  • Method for detecting biological membrane ageing state in sewage and wastewater processing system

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0036] In this embodiment, the detection of the aging state of the soft filler biofilm in a certain municipal sewage aerobic treatment system is carried out. The influent water quality of the system is: pH 7.17-7.85, chemical oxygen demand 340-520mg / L, ammonia nitrogen concentration 30-50mg / L, total nitrogen concentration 60-90mg / L, total phosphorus concentration 3.2-6.6 mg / L, conductivity 580~730μS / cm.

[0037] The specific operation for detecting whether the biofilm is aging is as follows:

[0038] Step 1. Take out the biofilm from the sewage treatment system: Take out the soft packing from the same depth of the sewage tank, cut the biofilm from the surface of the soft packing, put it in a -20°C freezing sampling box, and quickly bring it back for subsequent experiments ;

[0039]Step 2, biofilm pretreatment; use sterile water to make a 1000mg / L suspension of biofilm; take 2.5mL of biofilm suspension, add an equal volume of pre-cooled 0.6mol / L perchloric acid solution, and...

Embodiment 2

[0044] In this example, the detection of the aging state of the suspended filler biofilm in the comprehensive wastewater treatment system of a certain oxidation industrial park is carried out. The influent water quality of the system is: pH 7.08~7.52, chemical oxygen demand 1200~2540mg / L, ammonia nitrogen concentration 1.54~6.80mg / L, total nitrogen concentration 35~65mg / L, total phosphorus concentration 11.2~ 20.6mg / L, conductivity 12580~16340μS / cm.

[0045] The specific operation for detecting whether the biofilm is aging is as follows:

[0046] Step 1. Take out the biofilm from the sewage treatment system: After taking out the filler from the sewage tank, scrape the biofilm off the surface of the suspended filler, put it in a cryotube, freeze it in a liquid nitrogen bottle, and bring it back for experimental analysis;

[0047] Step 2, biofilm pretreatment; use sterile water to make a 200mg / L suspension of the biofilm; take 10mL of the biofilm suspension, add an equal volume...

Embodiment 3

[0052] In this example, the detection of the aging state of the suspended filler biofilm in a slightly polluted water source and aerobic water treatment system is carried out. The water quality of the system is: pH 7.66-8.35, chemical oxygen demand 14.3-19.6mg / L, ammonia nitrogen 0.88-2.91mg / L, UV 254 5.8~6.9m -1 , UV 254 The numerical value represents the ultraviolet absorbance under the unit cuvette light path at a wavelength of 254nm, reflecting the amount of humic macromolecular organic substances and aromatic compounds containing C=C double bonds and C=O double bonds naturally existing in water.

[0053] The specific operation for detecting whether the biofilm is aging is as follows:

[0054] Step 1. Take out the biofilm from the sewage treatment system: After taking out the filler from the sewage tank, scrape the biofilm off the surface of the suspended filler, place it in a -20°C frozen sampling box, and quickly bring it back to the laboratory;

[0055] Step 2, biofi...

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Abstract

The invention discloses a method for detecting the biological membrane ageing state in a sewage and wastewater processing system, and belongs to the technical field of water processing. The method includes the steps of firstly, conducting biological membrane sampling; secondly, conducting biological membrane preprocessing; thirdly, measuring the content of triphosadenine, adenosine diphosphate and adenosine monophosphate in a biological membrane; fourthly, calculating the adenylate energy charge of the biological membrane, and judging the ageing state of the biological membrane according to the numerical value of the adenylate energy charge, wherein the state is judged to be the ageing state if the numerical number is smaller than 0.6. The ageing state of the biological membrane can be judged directly through the adenylate energy charge, operation is easy and convenient, the result is visible, repeatability is high, the method is suitable for biological membranes of different sources, the efficiency of biological membrane method sewage processing operation monitoring can be remarkably improved, and normal running of a biological membrane method sewage processing system is ensured.

Description

technical field [0001] The invention belongs to the technical field of biofilm sewage treatment, and more specifically relates to a method for detecting the aging state of biofilm in a sewage treatment system. Background technique [0002] Biofilm method, also known as fixed membrane method, is a kind of wastewater biological treatment technology parallel with activated sludge method. It has strong adaptability to water quantity and quality, good removal effect on organic matter, good nitrification and denitrification functions, and small amount of sludge. , low power costs and other advantages, has been widely researched and applied for a long time. Biofilm is a structured community composed of multiple species in a self-generated extracellular network polymer matrix formed by microorganisms attached to the surface of the carrier (or filler), through the attachment growth on the surface of the carrier, mature and aging film shedding, and new film growth and other dynamic c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 黄辉任洪强丁丽丽
Owner NANJING UNIV
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