Method of quickly measuring content of Paenibacillus polymyxa in compound microbial agent

Abstract

The invention discloses a method of quickly measuring content of Paenibacillus polymyxa in a compound microbial agent. Active ingredients of the compound microbial agent include Bacillus subtilis, Paenibacillus polymyxa and Bacillus mucilaginosus. Ampicillin sodium of certain concentration is added into a culture medium to inhibit the growth of Bacillus subtilis but not inhibit the growth of Paenibacillus polymyxa, thereby avoiding the influence of quickly grown Bacillus subtilis upon statistic data of Paenibacillus polymyxa. The method allows the content of Paenibacillus polymyxa in the compound microbial agent to be measured quickly, simply and accurately.

Description

technical field [0001] The invention relates to a detection method for a composite microbial agent, in particular to a method for quickly measuring the content of Paenibacillus polymyxa in a composite microbial agent, and belongs to the technical field of microbial detection. Background technique [0002] Microbial fertilizers, commonly known as bacterial agents, referred to as bacterial agents, refer to a type of specific fertilizer containing live microorganisms, which can obtain specific fertilizer effects when used in agricultural production. After application, through the life activities of microorganisms in the bacterial agent, with the help of their metabolic processes or metabolites, the growth conditions of plants, especially the nutritional environment, are improved. In order to improve fertilizer efficiency, several live microorganisms are often mixed in the bacterial agent. [0003] Microorganisms such as Paenibacillus polymyxa, Bacillus subtilis and Bacillus je...

AI Technical Summary

Problems solved by technology

Bacillus jelly-like, also known as silicate bacteria, although it does not require high nutritional conditions and has strong adaptability to environmental conditions, usually the amount of Bacillus jelly-like added to the compound bacterial agent is only The amount of Bacillus or Paenibacillus polymyxa is several tenths or even one percent. Therefore, when the compound bacterial agent is diluted to the corresponding multiple to determine the content of Bacillus subtilis and Paenibacillus polymyxa, even if the jelly-like Bacillus can Grow under this culture condition, but the amount of bacteria it grows will not cause significant impact on the statistical results of Bacillus subtilis and Paenibacillus polymyxa, so when measuring, often do not consider the amount of jelly-like bacillus; The determination of frozen-like Bacillus is relatively easy, because it has its unique colony and cell morphological characteristics on a selective (no nitrogen or no effective phosphorus and potassium nutrient) solid medium, so it is easy to detect without biochemical characteristics or gene sequence analysis. Easily distinguished from other bacteria

However, the medium used by Bacillus subtilis and Paenibacillus polymyxa is the same. Under conventional culture conditions, Bacillus subtilis grows faster, while Paenibacillus polymyxa grows relatively slowly, and grows rapidly in the medium. The Bacillus subtilis covers the Paenibacillus polymyxa, which makes it impossible to accurately count the content of the Paenibacillus polymyxa, and the adaptability of the Bacillus subtilis is more than that of the Paenibacillus myxa, and it is difficult to accurately control the culture temperature and other simple methods. Count the content of Paenibacillus polymyxa

There is no literature report on the inhibition of Bacillus subtilis or Paenibacillus polymyxa by ampicillin sodium

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