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Preparation method of environmental protection enzyme and algal bloom inhibitor

An environmentally friendly enzyme, enzyme technology, applied in herbicides and algaecides, botanical equipment and methods, biochemical equipment and methods, etc., to achieve the effect of water quality improvement, good water quality, and help the recovery of the water body itself

Inactive Publication Date: 2019-01-01
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are very few studies on the application of environmental protection enzymes on the suppression of algae blooms in eutrophic lakes

Method used

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  • Preparation method of environmental protection enzyme and algal bloom inhibitor
  • Preparation method of environmental protection enzyme and algal bloom inhibitor
  • Preparation method of environmental protection enzyme and algal bloom inhibitor

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Experimental program
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preparation example Construction

[0049] The invention also discloses a preparation method of an environment-friendly enzyme, comprising the following steps:

[0050] Fruits and / or vegetables, water and sugars are put into airtight containers in proportion to ferment for 3-6 months, and the clear liquid after filtration is filtered to obtain the environment-friendly enzyme.

[0051] Wherein, the fermented raw materials may only contain fruits, not vegetables; for example, preferably 1 / 3 apple peel, 1 / 3 banana peel and 1 / 3 orange peel. The fermented raw materials can also only contain vegetables without fruits; for example, 1 / 3 of lettuce, 1 / 3 of cabbage and 1 / 3 of spinach are preferred.

[0052] Wherein, the environment-friendly enzyme is mixed and fermented according to the volume ratio of fruits and / or vegetables: water: sugar = 1: 2-4: 8-12.

[0053] The invention also discloses a preparation method of an algal bloom inhibitor, comprising the following steps:

[0054] The algal bloom inhibitor is obtained...

Embodiment 1

[0064] Embodiment 1 environmental protection enzyme is to the inhibition of Microcystis aeruginosa

[0065] (1) Experimental supplies

[0066] Microcystis aeruginosa, enzymes;

[0067] Medium: sodium nitrate, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride, sodium carbonate, ferric citrate, Na 2 EDTA;

[0068] No ammonia: concentrated sulfuric acid;

[0069] Total nitrogen: potassium nitrate, sodium hydroxide, potassium persulfate, concentrated hydrochloric acid;

[0070] Total phosphorus: ascorbic acid, ammonium molybdate, potassium antimony tartrate, potassium dihydrogen phosphate;

[0071] Ammonia nitrogen: ammonium chloride, potassium sodium tartrate, potassium iodide, mercury dichloride, potassium hydroxide;

[0072] Chemical oxygen demand: potassium dichromate, silver sulfate, ferrous sulfate, ammonium ferrous sulfate, 1,10-phenanthroline, mercury sulfate;

[0073] 25mL glass colorimetric tube with stopper, 50mL glass colorimetric tube with stop...

Embodiment 2

[0122] Embodiment 2 environmental protection enzyme is to the inhibition of the growth of Scenedesmus tetrascaus and the improvement effect to water quality

[0123] The prepared environmental protection enzyme was added dropwise to the culture solution of Scenedesmus tetrascaus and a blank control was set, with three parallels for each group.

[0124] On the third day of the experiment, the flocculation and sedimentation of Scenedesmus tetrascaus to the bottom of the medium can be clearly observed in the algae liquid medium added with 1:240, 1:120 and 1:60 concentrations of environmental protection enzymes, and the higher the concentration of the enzyme added in the medium , the less amount of flocculation and sedimentation of Scenedesmus tetracerus. The water in the upper part of the algae liquid medium with 1:240 concentration of environmental protection enzyme becomes clear and transparent; the algae liquid medium with 1:120 and 1:60 concentration of environmental protecti...

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Abstract

A preparation method for garbage enzyme comprises the following steps: putting fruits and / or vegetable, as well as water and saccharides into a closed container according to a ratio, fermenting for 3 to 6 moths, filtering, and taking clear liquid after the filtering, thus obtaining the garbage enzyme, wherein the garbage enzyme is formed by mixing and fermenting according to the volume ratio that the fruits and / or the vegetables : the water : the saccharides is equal to 1: (2 to 4) : (8 to 12). A preparation method for an algal bloom inhibitor comprises the following step: adding water to dilute the garbage enzyme prepared by the preparation method for the garbage enzyme, thus obtaining the algal bloom inhibitor. The algal bloom inhibitor has a remarkable effect of inhibiting alga growth, contributes to aquatic ecological environment purification and water body self-recovery, and has a perfect improving effect on water quality; the preparation methods for the garbage enzyme and the algal bloom inhibitor are simple, low in cost, easy for industrialized operation, and capable of avoiding secondary pollution.

Description

technical field [0001] The invention relates to the field of ecological restoration of water body environment, and more specifically relates to a preparation method of an environmental protection enzyme and an algal bloom inhibitor. Background technique [0002] Lakes are the main source of water for agriculture, aquaculture, and domestic use. They also have the functions of maintaining biodiversity, regulating climate, storing floods, regulating surface runoff, and purifying water quality. With the rapid development of my country's economy, the urban population continues to increase, the discharge of industrial and agricultural wastewater and domestic sewage continues to increase, a large amount of nutrients continue to enter the lake, the proportion of eutrophic lakes increases year by year, and the abnormal proliferation of algae occurs frequently, forming water blooms . The dominant algae of lake water blooms are generally cyanobacteria, green algae and diatoms, among wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/00A01N63/00A01P13/00A01P1/00C02F1/50
Inventor 杨苏文
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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