A method for preparing molecularly imprinted polymers that recognize sulfonamide antibiotics

A molecular imprinting and polymer technology, applied in the field of pollutant detection, separation and purification, can solve problems such as unfavorable sulfonamide antibiotic identification methods, sulfonamide antibiotic identification, etc., to improve adsorption performance, enhance mechanical properties, and enhance application potential. Effect

Active Publication Date: 2019-03-05
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, molecular imprinting technology has been widely used in the field of purification of antibiotics as a sample pretreatment method. For the specific recognition material of a certain sulfa antibiotic, there is no universal molecularly imprinted polymer material for the recognition of sulfa antibiotics. Therefore, when identifying sulfa antibiotics, it is necessary to prepare a variety of molecularly imprinted materials for the target. Conducive to the establishment of a simple and universal identification method for sulfonamide antibiotics

Method used

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  • A method for preparing molecularly imprinted polymers that recognize sulfonamide antibiotics
  • A method for preparing molecularly imprinted polymers that recognize sulfonamide antibiotics
  • A method for preparing molecularly imprinted polymers that recognize sulfonamide antibiotics

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Effect test

Embodiment 1

[0018] (1) Prepolymerization of template molecules: dissolving sulfonamide, 4-vinylpyridine and acrylamide in acetonitrile according to the molar ratio of 1:2:2, stirring at room temperature to form a homogeneous transparent solution;

[0019] (2) Immobilization of template molecules: according to the ratio of trimethylolpropane trimethacrylate: sulfonamide molar ratio of 10:1, add trimethylolpropane trimethacrylate to the solution of step (1), stir at room temperature After dissolving, add 20mg of azobisisobutyronitrile to the solution, pass through nitrogen gas to deoxygenate, after sealing, place the solution in a water bath at 60°C for 12 hours;

[0020] (3) Template molecule elution: collect the supernatant and precipitate; use a UV spectrophotometer to test the absorbance of sulfonamide in the supernatant; add the eluent to the centrifuged precipitate, stir and reflux at 100°C for 30min, After centrifugation, collect the precipitate and supernatant respectively, and repe...

Embodiment 2

[0023] (1) Prepolymerization of template molecules: dissolving sulfonamide, 4-vinylpyridine and acrylamide in toluene at a molar ratio of 1:1.5:1.5, and stirring at room temperature to form a homogeneous transparent solution;

[0024] (2) Polymerization: According to the ratio of trimethylolpropane trimethacrylate: sulfonamide molar ratio of 15:1, add trimethylolpropane trimethacrylate to the solution in step (1), stir at room temperature until dissolved , and then add 30 mg of azobisisobutyronitrile to the solution, pass through nitrogen gas to remove oxygen, after sealing, place the solution in a water bath at 80°C for 12 hours;

[0025] (3) Template molecule elution: the solution obtained in step (2) is centrifuged, and the supernatant and precipitate are collected; the absorbance of sulfonamide in the supernatant is checked by a UV spectrophotometer; The eluate was stirred and refluxed at 100°C for 60 minutes, and the precipitate and supernatant were collected after centri...

Embodiment 3

[0028] (1) Template molecule prepolymerization: Dissolve sulfonamide (template molecule), 4-vinylpyridine and acrylamide in methanol at a molar ratio of 1:1:1, and form a homogeneous transparent solution after stirring at room temperature;

[0029] (2) Immobilization of template molecules: according to the ratio of trimethylolpropane trimethacrylate: sulfonamide molar ratio of 20:1, add trimethylolpropane trimethacrylate to the solution of step (1), stir at room temperature After dissolving, add 40mg of azobisisobutyronitrile to the solution, pass through nitrogen gas to deoxygenate, after sealing, place the solution in a water bath at 70°C for 24 hours;

[0030] (3) Template molecule elution: the solution obtained in step (2) is centrifuged, and the supernatant and precipitate are collected; the absorbance of sulfonamide in the supernatant is checked by a UV spectrophotometer; The eluate was stirred and refluxed at 120°C for 30 minutes, and the precipitate and supernatant wer...

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Abstract

The invention discloses a preparation method of molecularly imprinted polymer capable of recognizing sulfonamide antibiotics, and particularly relates to a preparation method of an antibiotic recognition material with universality. The molecularly imprinted polymer is prepared by adopting a method of precipitation polymerization, taking sulfonamide antibiotics as a template, taking acrylamide and 4-vinylpyridine jointly as a functional monomer and taking trimethylol propyl triacrylate as a crosslinking agent. The sulfonamide antibiotics can be quickly recognized by the molecularly imprinted polymer prepared by the method, status quo that the sulfonamide antibiotics are deficient is solved, and the molecularly imprinted polymer has application potential in quick detection of the sulfonamide antibiotics and sample pretreatment.

Description

technical field [0001] The invention belongs to the field of pollutant detection, separation and purification, and relates to a preparation method of a molecularly imprinted polymer for identifying sulfonamide antibiotics in the environment. Background technique [0002] Sulfonamide antibiotics are widely used synthetic antibiotics, which have the characteristics of hydrophilicity and strong stability, and can easily enter the water environment and aquatic products. Long-term drinking or consumption of water or aquatic products with residual sulfonamide antibiotics by humans will cause symptoms such as allergic reactions, cancer, deformity and drug resistance in the body. At present, studies have reported that high concentrations of sulfonamide antibiotics have been detected from aquatic products, surface water, and influent and effluent water from sewage treatment plants. Therefore, it is necessary to develop a universal material that can identify sulfonamide antibiotics. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F222/14C08F226/06C08F220/56C08J9/26B01J20/26B01J20/30
Inventor 胡玉峰王成李想道陈涛臧新科赵雅彤柳丽芬
Owner DALIAN UNIV OF TECH
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