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New application of luteolin

A technology for the application of luteolin, which is applied in the new application field of luteolin, and can solve the problems such as the antiviral effect of luteolin that have not been reported

Inactive Publication Date: 2015-12-30
JILIN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the antiviral effects of luteolin on Enterovirus 71 and Coxsackievirus A16 have not been reported yet.

Method used

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  • New application of luteolin
  • New application of luteolin
  • New application of luteolin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Cytotoxicity of luteolin to cells and inhibition of cytopathic effects caused by Enterovirus 71 and Coxsackievirus A16

[0023] Human rhabdomyosarcoma cells in the logarithmic growth phase (RD cells, purchased from ATCC, accession number: #CCL-136) and RD cells stably expressing the EV71 / CA16 receptor hSCARB2 gene (NCBI accession number: NM_005506.3) RDS cells were obtained from the National Engineering Laboratory of AIDS Vaccine, Jilin University. For the construction method, see Lietal. 4 pcs / hole and 6×10 4 The concentration of cells / well was inserted into a 96-well culture plate and cultured for 24 hours. Both RD cells and RDS cells were incubated at 37 °C and 5% CO 2 The medium used for culturing RD cells was DMEM medium supplemented with 10% fetal bovine serum (abbreviated as 10% FBS-DMEM, both FBS and DMEM were purchased from Sigma), and the medium used for cultivating RDS cells was containing 0.5 μg / ml puromycin (purchased from Clontech) in 10% FBS...

Embodiment 2

[0039] Example 2: Inhibitory effect of luteolin on plaque formation caused by EV71 and CA16

[0040] Take the RDS cells in the logarithmic growth phase, and use 6×10 5 The concentration of each well was inserted into a 12-well culture plate and cultured for 24 hours.

[0041] Aspirate the medium in each well, add 300 μL of 2% FBS-DMEM cell culture solution containing different concentrations of luteolin, so that the final concentration of luteolin in the well is 50 μM, 25 μM, 12.5 μM, 6.25 μM, each concentration is set to 3 Multiple wells; add 300 μ LEV71 or CA16 (100 PFU / hole) virus to each well while adding medicine to infect; and set only adding 2% FBS-DMEM cell culture medium cell control and only adding EV71 or CA16 to infect the virus control; After continuing to culture the cells for 1 hour, suck off the culture supernatant and rinse the cells twice with sterile PBS, add 1% agar 2% FBS-DMEM medium containing corresponding concentrations of luteolin to the drug-dosed gr...

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Abstract

The invention relates to new application of luteolin, in particular to application of luteolin in preparing medicine for preventing and treating hand-foot-mouth diseases, particularly, medicine used for resisting enterovirus 71 and / or coxsackievirus A16.

Description

technical field [0001] This application relates to the new application of luteolin, in particular, to the application of luteolin for the preparation of medicines for the prevention and treatment of hand, foot and mouth disease, especially for the preparation of anti-enterovirus type 71 and / or Coxsackie Use of virus A16 drug. Background technique [0002] HFMD is a common infectious disease in children. The main pathogens are Enterovirus 71 (abbreviated as EV71) and Coxsackievirus A16 (abbreviated as CA16). In recent years, frequent outbreaks of HFMD in the Asia-Pacific region have become a serious social health problem. According to statistics, from 2008 to 2012 in my country, more than seven million cases of hand, foot and mouth disease were reported, resulting in 2443 deaths. [0003] There is currently no commercially available vaccine to prevent HFMD. In terms of treatment, conventional antiviral drugs such as acyclovir, ganciclovir, and ribavirin are often used in c...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P31/14
Inventor 苏维恒姜春来麻彤辉孔维徐琳
Owner JILIN UNIV
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