Method of testing amentoflavone in preparation of bone tissue engineering seed cells
A kind of technology of biflavone and bone tissue engineering, which is applied in the experimental field of biflavone of spiconia in the preparation of seed cells for bone tissue engineering, to achieve the effect of promoting early osteogenic differentiation, promoting MSCs proliferation, and promoting late osteogenic differentiation
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[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0026] A test method for preparing seed cells of bone tissue engineering with biflavonoids.
[0027] (1) MSCs culture
[0028] MSCs were placed in α-MEM medium containing 10% fetal bovine serum at 37°C, 5% CO 2 Cultivate in the incubator, change the medium once every 2-3 days, when the cells reach 80% confluence, digest and subculture with 0.25% trypsin;
[0029] (2) Cell proliferation detection
[0030] MSCs cells were seeded in 96-well culture plates, and...
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