Composite underleaf pearl preparation and application in dietary for dog
A compound and preparation technology, applied in the field of compound Phyllostachys phylloxera preparations, achieves the effects of high development value, simple extraction and separation process, and convenient production
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Embodiment 1
[0017] The preparation of embodiment 1 compound phylloxera preparation
[0018] Every 5g of the whole plant under the leaf is crushed, add 150mL of distilled water and stir evenly, soak for 16h, decoct for 45min in a slightly boiling state, and filter off the supernatant. Add 150 mL of distilled water to the filter residue, soak for 8 hours, decoct in a slight boiling state for 35 minutes, and filter off the supernatant. Add 150 mL of distilled water to the filter residue, soak for 8 hours, decoct in a slight boiling state for 35 minutes, and filter off the supernatant. The combined three filtrates were concentrated under reduced pressure to 40 mL. The same method is used to decoct the roots of Sansevieria verticillium, combine the extracts of Phyllanthus phylloxera and Rhizoma Sansevieriae, further concentrate to 40mL, and store at 4°C for later use.
Embodiment 2
[0019] Example 2 Effect of compound phylloxera preparation on liver function index of carbon tetrachloride liver injury in dogs
[0020] 1 Materials and methods
[0021] 1.1 Test materials and instruments
[0022] Carbon tetrachloride: batch number 20140218, manufacturer Sinopharm Chemical Reagent Co., Ltd.
[0023] Rapeseed oil: Arowana rapeseed oil was purchased from Yonghui Supermarket in Fuzhou City.
[0024] Bifendate dripping pills: Approval number Guoyao Zhunzi H11020980, manufacturer Beijing Concord Pharmaceutical Factory.
[0025] The dried whole plant of Phyllostachys phylloxera and Rhizoma chinensis root were purchased in Zhangzhou, Fujian, and were identified as Phyllostachys phylloxera and Rhizoma chinensis.
[0026] Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALKP) kits were provided by Nanjing Jiancheng Bioengineering Institute.
[0027] 800D desktop vulgar centrifuge (Shanghai Medical Instrument Co., Ltd. Surg...
Embodiment 3
[0060] Example 3 Effect of Compound Phyllostachys phylloxera preparation on the antioxidant index of carbon tetrachloride liver injury in dogs
[0061] 1 Materials and methods
[0062] 1.1 Test materials and instruments
[0063] Carbon tetrachloride: batch number 20140218, manufacturer Sinopharm Chemical Reagent Co., Ltd.
[0064] Rapeseed oil: Arowana rapeseed oil was purchased from Yonghui Supermarket in Fuzhou City.
[0065] Bifendate dripping pills: Approval number Guoyao Zhunzi H11020980, manufacturer Beijing Concord Pharmaceutical Factory.
[0066] The dried whole plant of Phyllostachys phylloxera and Rhizoma chinensis root were purchased in Zhangzhou, Fujian, and were identified as Phyllostachys phylloxera and Rhizoma chinensis.
[0067] Glutathione (GSH), malondialdehyde (MDA), and total superoxide dismutase (T-SOD) kits were all provided by Nanjing Jiancheng Bioengineering Institute.
[0068] 800D desktop vulgar centrifuge (Shanghai Medical Instrument Co., Ltd...
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