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Method for high yield production of aerobic single-cell protein by autolysis process

A single-cell protein, high-yield technology, applied in the direction of microbial/single-cell algae protein processing, etc., can solve the problems of not considering the decomposition of nucleic acid into high-value nucleotides, failing to reach the average production capacity of processing equipment, and low protein digestibility , to achieve the effect of controllable residence time, prevention of diarrhea, and rapid heating

Inactive Publication Date: 2015-12-23
TAIZHOU ICELL BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing process is directly dried and microwave sterilized after dehydration, which destroys most of the endogenous enzymes, so no active substances will be produced;
[0007] 3) The protein digestibility of the existing processing technology is still not high, and there is still room for further improvement;
[0008] 4) The single-cell protein has a relatively high content of nucleic acid. The drying treatment of the ordinary process does not decompose the nucleic acid into nucleotides and remains in the cell. However, decomposing the nucleic acid into nucleotides by a certain method can make the single-cell protein be applied to animals. Feed has two obvious effects of attracting food and immunity. The existing technology does not consider how to decompose nucleic acid into high-value nucleotides
[0009] 5) Although the yield of cultured single-cell protein is very high, the production capacity of subsequent processing equipment can still be significantly higher than that of cultured single-cell protein. For example, the production capacity of the processing equipment of the invention patent CN2014100556582 is limited by the low yield of culture output Unable to reach the maximum capacity, or even the average capacity of the processing equipment
However, these two patents are not suitable for this patent. The raw materials used in this patent application are anaerobic bacteria. The characteristics of bacteria are quite different from those of aerobic bacteria. The autolysis methods are also very different. Long, need to add enzymes to promote autolysis, anaerobic bacteria autolysis has more restrictions on the reaction vessel, moisture content, pH, temperature, etc., and the product is difficult to control, and it is easy to produce a high content of spoilage products

Method used

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  • Method for high yield production of aerobic single-cell protein by autolysis process
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  • Method for high yield production of aerobic single-cell protein by autolysis process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1: the establishment of free nucleotide and free base detection method

[0054] Use free nucleotides and free bases as the main indicators for the adjustment of autolysis process parameters and the comparison of products before and after autolysis. The principle is: raw materials such as yeast and bacterial protein will contain free nucleotides and bases after autolysis or enzymatic hydrolysis, and the sample extracted from the aqueous solution will be analyzed by high-performance liquid chromatography. Salt is used as the mobile phase, and the external standard method can be used for quantitative determination of 5 nucleotides, 5 nucleosides and 5 bases.

[0055] Free nucleotides refer to the sum of 5 nucleotides and 5 nucleosides, including cytidylic acid, uridine acid, guanylic acid, inosinic acid, adenylic acid, cytidine, uridine, inosine, guanylic acid glycosides, adenosine. Free base refers to the sum of 5 nucleic acid bases, including cytosine, uraci...

Embodiment 2

[0119] Embodiment 2: autolysis production technology and product

[0120] The autolysis production process and products developed by the present invention are single-cell protein products made by using the cultivation technology of the invention patent WO2009059163A1, but are not limited thereto, and are also applicable to the processing of aerobic single-cell protein products similar to this invention.

[0121] A high-yield processing method of autolyzed single-cell protein is realized by dividing the processing process into two parts, which are respectively completed by different production units. The first part is the pre-processing unit, which includes the process from precipitation concentration to centrifugal dehydration after the single-cell protein culture is completed. In this part, multiple preprocessing units can be set, for example, 2 to 10 units are all possible. The second part is the post-processing unit, which includes the operations of autolysis, drying and s...

Embodiment 3

[0162] Embodiment 3: digestibility test

[0163] The difference in apparent digestibility between the bacterial protein using the autolysis process and the common bacterial protein was evaluated by animal experiments.

[0164] Digestibility is the percentage of digestible nutrients in the feed ingredients ingested by animals. The indicator method is to use the indicator existing in the feed or artificially mixed evenly. After feeding the fish, the digestibility was calculated from the amount of the unit indicator contained in the unit feed and feces. Formulating formulas based on the digestible value of nutrients in feed ingredients is of great significance for improving the digestibility of compound feed and reducing the pollution of feed substances to the aquaculture water environment.

[0165] Test plan:

[0166] Raw materials for evaluation: The bacterial protein with a protein content of 50% was autolyzed according to the method of Example 2, and the same batch of sing...

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Abstract

The invention relates to the technical field of single-cell protein processing, in particular to a method for high yield production of aerobic single-cell protein by an autolysis process. The process is characterized in that in each pre-processing unit, the material obtained by a centrifugal dewatering step is transported to a post-processing unit to conduct centralized treatment, and the post-processing unit consists of an autolysis step, a drying step, a sterilization step and a crushing and packaging step. The technology and the prepared product involved in the invention employ the autolysis process, during autolysis, autolytic enzyme fully decomposes macromolecular nutrients and cell walls, autolysis can produce high content free amino acid and free nucleotide, and at the same time cell wall polysaccharides are decomposed, and cells can produce permeability so as to ensure that active substances can flow out of cells to as to be directly absorbed and utilized by animals. Autolysis is carried out after centrifugal dewatering, high efficiency of autolysis can be ensured, and the content of water needing to be removed in a drying process after autolysis can be reduced, thereby reducing the cost of drying.

Description

[technical field] [0001] The invention relates to the technical field of single-cell protein processing, in particular to a method for producing aerobic single-cell protein by using an autolysis process with high yield. [Background technique] [0002] Single cell protein (SCP for short), also known as microbial protein or bacterial protein, uses industrial wastewater, waste gas, natural gas, petroleum alkanes, agricultural and sideline processing products, and organic waste as culture media to cultivate yeast and non-pathogenic bacteria. , Microbacteria, fungi and other single-cell organisms, and then made after purification and drying treatment, it is an important protein source for the food industry and feed industry. Single-cell protein products are rich in protein, with a complete range of amino acids and appropriate proportions. Single-cell protein is also rich in fat, carbohydrates, nucleic acids, vitamins and inorganic salts, and contains calcium, phosphorus, potassi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/20
Inventor 许建刚宋建华赵维韦张鑫杰
Owner TAIZHOU ICELL BIO TECH CO LTD
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