Preparation method for low-purine edible fungus vinegar rich in amino acid and vitamin D
A technology of edible mushrooms and amino acids, applied in the field of beverage processing, can solve the problems of vitamin D-free and high purine content, achieve high vitamin D content, unique taste, and avoid degradation
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[0024] (1) 2Kg of fresh mushrooms are crushed with a mushroom grinder, mixed with 6.0L of water and crushed by ultrasonic waves. The ultrasonic crushing time of 100mL mixed solution is 30s, with an interval of 30s. The total crushing time is 5 minutes, and the ultrasonic crushing strength is 50%. Hour, obtain about 6L mushroom broken liquid.
[0025] (2) Preparation of Arthrobacter ATCC21606 disruption solution: the culture medium for Arthrobacter is K 2 HPO 4 1.5g / L, KH 2 PO40.5g / L, MgSO 4 0.5g / L, FeSO 4 1mg / L, xanthine 0.01M / L, adenine, hypoxanthine, guanine each 0.005M / L, pH7.0; cultivate Arthrobacter ATCC21606 to the concentration of OD 600 =5.0, first use high-pressure crushing directly, the crushing pressure is 20000psi; then ultrasonic crushing, the bacterial liquid volume is 100mL, the ultrasonic crushing time is 20s, the interval is 20s, the total crushing time is 5 minutes, and the ultrasonic crushing strength is 50%.
[0026] (3) Add Arthrobacter crushing solut...
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