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Method for increasing yield of dihydromyricetin in ampelopsis grossedentata

A technology of Snake-toothed Grape and dihydromyricetin, which can be used in pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of high cost and low efficiency of dihydromyricetin extraction, and improve the market Supply, Increased Extraction Yield, Effect of Economic Extraction

Active Publication Date: 2015-12-16
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The present invention aims at the problems of high cost and low efficiency of extracting dihydromyricetin from the current Snake Grape Stems and leaves are used as raw materials. Using this method to extract dihydromyricetin, the efficiency is much higher than that of natural shade drying and ordinary drying samples, avoiding the metabolic loss of dihydromyricetin, and greatly reducing the extraction cost, achieving dihydromyricetin The efficient and economical extraction of myricetin will have a profound impact on meeting the market supply of dihydromyricetin and reducing the raw material price of dihydromyricetin, which has important practical significance

Method used

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  • Method for increasing yield of dihydromyricetin in ampelopsis grossedentata
  • Method for increasing yield of dihydromyricetin in ampelopsis grossedentata
  • Method for increasing yield of dihydromyricetin in ampelopsis grossedentata

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Experimental program
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Effect test

Embodiment 1

[0041] The method for improving the yield of dihydromyricetin in Snake dentata grape comprises the following steps:

[0042] 1) Picking fresh leaves from the experimental field of Snake-tooth grape in Wuhan, Hubei, and baking the leaves of Snake-toothed grape at a temperature of 80°C for 2 hours;

[0043] 2) pulverize the dry-processed Snake Grapes, pass through a 40-mesh sieve to obtain Snake Grapes powder, and set aside;

[0044] 3) Weighing 100g of Eperodonta grape powder and adding it to 2000mL ethanol solution with a volume fraction of 75%, ultrasonically extracting for 30min, and filtering to obtain dihydromyricetin extract;

[0045] 4) Put the dihydromyricetin extract prepared in step 3) in a rotary evaporator, concentrate under reduced pressure in a water bath at a temperature of 50°C, recover ethanol until it has no alcohol smell, add 1000mL of boiling water to dissolve, and put it in a low temperature environment at 4°C. Cold crystallization, filtration, and drying ...

Embodiment 2

[0047] The method for improving the yield of dihydromyricetin in Snake dentata grape comprises the following steps:

[0048] 1) Picking fresh leaves from the experimental garden of Snake-tooth grape in Wuhan, Hubei, and baking the leaves of Snake-toothed grape at a temperature of 100°C for 1.5 hours;

[0049] 2) pulverize the dry-processed Snake Grapes, pass through a 40-mesh sieve to obtain Snake Grapes powder, and set aside;

[0050] 3) Weighing 100g of Eperodonta grape powder and adding it to 2000mL ethanol solution with a volume fraction of 75%, ultrasonically extracting for 30min, and filtering to obtain dihydromyricetin extract;

[0051] 4) Put the dihydromyricetin extract prepared in step 3) in a rotary evaporator, concentrate under reduced pressure in a water bath at a temperature of 50°C, recover ethanol until it has no alcohol smell, add 1000mL of boiling water to dissolve, and put it in a low temperature environment at 4°C. Cold crystallization, filtration, and dry...

Embodiment 3

[0053] The method for improving the yield of dihydromyricetin in Snake dentata grape comprises the following steps:

[0054] 1) Picking fresh leaves from the experimental field of Snake-tooth grape in Wuhan, Hubei, and baking the leaves of Snake-tooth grape at a temperature of 120°C for 1 hour;

[0055] 2) pulverize the dry-processed Snake Grapes, pass through a 40-mesh sieve to obtain Snake Grapes powder, and set aside;

[0056] 3) Weighing 100g of Eperodonta grape powder and adding it to 2000mL ethanol solution with a volume fraction of 75%, ultrasonically extracting for 30min, and filtering to obtain dihydromyricetin extract;

[0057] 4) Put the dihydromyricetin extract prepared in step 3) in a rotary evaporator, concentrate under reduced pressure in a water bath at a temperature of 50°C, recover ethanol until it has no alcohol smell, add 1000mL of boiling water to dissolve, and put it in a low temperature environment at 4°C. Cold crystallization, filtration, and drying of...

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Abstract

The invention discloses a method for increasing the yield of dihydromyricetin in ampelopsis grossedentata. The method includes roasting ampelopsis grossedentata medicinal materials under the condition of temperatures of 80-120 DEG C for 1-2h; smashing the ampelopsis grossedentata medicinal materials to obtain ampelopsis grossedentata powder; uniformly mixing the ampelopsis grossedentata powder and ethyl alcohol with each other to obtain mixtures; ultrasonically extracting the mixtures and filtering the mixtures to obtain dihydromyricetin extract liquid; concentrating the dihydromyricetin extract liquid under reduced-pressure conditions, chilling and crystallizing the dihydromyricetin extract liquid in low-temperature environments, and filtering and drying the dihydromyricetin extract liquid to obtain filter cake which is a dihydromyricetin extract. The method has the advantages that the yield of the dihydromyricetin in samples obtained by means of high-temperature drying treatment is obviously higher than the yields of dihydromyricetin in samples dried in shade and normal dried samples, metabolism loss of the dihydromyricetin can be prevented, the extraction cost can be greatly lowered, the method is easy and convenient to implement and high in feasibility and is a method for efficiently and economically extracting the dihydromyricetin, the dihydromyricetin production efficiency can be obviously improved, the production cost can be lowered, and reasonable utilization of traditional Chinese medicine resources can be promoted.

Description

technical field [0001] The invention relates to the technical field of extraction of effective components of traditional Chinese medicines, in particular to a method for increasing the yield of dihydromyricetin in the velociraptor grape. Background technique [0002] Ampelopsis grossedentata (Hand-Mazz) W.T.wang is a perennial woody vine of the genus Ampelopsis grossedentata, widely distributed in Hunan, Hubei, Guizhou, Guangxi, Fujian and other places south of the Yangtze River in my country (Illustrated Book of Higher Plants of China (two volumes) Supplement, Science Press, 1983). The young stems and leaves are mostly picked by the folks and processed into tea-like products, commonly known as rattan tea, Maoyan berry tea, and Dragon Boat Festival tea. It tastes slightly sweet, bitter, and cool in nature. It has the effects of clearing away heat and detoxification, promoting dampness and reducing swelling, calming the liver and lowering blood pressure, promoting blood circu...

Claims

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Application Information

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IPC IPC(8): C07D311/32C07D311/40A61K36/87
CPCA61K36/87A61K2236/333A61K2236/51C07D311/32C07D311/40
Inventor 施春阳方建国王文清王丹丹熊微陈婧刘旭侯小龙汪秋兰
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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