Single microalgae cell activity dynamic monitoring novel method and device based on gas-liquid interface single cell capture and chlorophyll fluorescence characterization

Abstract

The invention discloses a single microalgae cell activity dynamic monitoring deviceand method based on gas-liquid interface single cell capture and chlorophyll fluorescence characterization. The device comprises a single microalgae cell capture unit and a single microalgae cell activity dynamic monitoring unit; the single microalgae cell capture unit comprises a bubble generator and a micro-fluidic chip component; the method comprises the following steps: generating a bubble near a micro-fluidic chip capture area; injecting a microalgae cell suspension into a channel of a micro-fluidic chip, and enabling a single microalgae cell to adsorb on the bubble through collision; detecting a captured single microalgae cell. According to the device and method, a method of laser-induce chlorophyll fluorescence is adopted for detecting the activity of a static microalgae cell, so that the defects brought by a method pproach of coloring a microalgae cell sample before detection in the prior art are overcome, and the device and method have the characteristics of less workload and higher accuracy.

Description

technical field [0001] The invention relates to a dynamic monitoring technology for single algae cell activity based on air-liquid interface single cell capture and chlorophyll fluorescence characterization, in particular to a new method and device for dynamic monitoring of single algae cell activity based on air-liquid interface single cell capture and chlorophyll fluorescence characterization. Background technique [0002] Microalgae are a class of ancient lower plants, widely distributed in oceans, freshwater lakes and other waters. In recent years, the invasion of alien organisms that spread with the ship's ballast water has caused serious environmental pollution and huge economic losses worldwide. Microalgae, as one of the main alien organisms, have also received more and more attention. . Moreover, microalgae cells contain a variety of high-value nutrients and chemical raw materials, and more and more algae have been used in experiments. At present, the methods used ...

AI Technical Summary

Problems solved by technology

Its disadvantages are: it is powerless to measure the volume of microalgae, and the required equipment is expensive and cumbersome. It is basically used to collect samples and take them back to the laboratory for culture and identification, which is time-consuming, laborious, inefficient, and the types of detection are relatively limited.

[0013] These cell capture methods often require external complex equipment, sample pretreatment is cumbersome, and the operation steps are complicated. It cannot capture a single algal cell quickly and conveniently, which is not conducive to the subsequent detection of the activity of a single microalgal cell.

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