Promoter for regulating and controlling expression of gene in glandular trichomes and application of promoter

A technology for regulating genes and promoters, applied in applications, genetic engineering, plant gene improvement, etc., can solve problems such as the undiscovered AaPDR1 gene promoter sequence

Active Publication Date: 2015-12-09
唐克轩 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After searching the prior art documents, it is found that there is no report related to the AaPDR1 gene promoter sequence of the present invention

Method used

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  • Promoter for regulating and controlling expression of gene in glandular trichomes and application of promoter
  • Promoter for regulating and controlling expression of gene in glandular trichomes and application of promoter
  • Promoter for regulating and controlling expression of gene in glandular trichomes and application of promoter

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Experimental program
Comparison scheme
Effect test

Embodiment

[0039] This embodiment relates to the acquisition of the AaPDR1 gene promoter, which specifically includes the following steps:

[0040] Step 1, Cultivate Artemisia annua sterile vaccine

[0041] Artemisia annua seeds were soaked in ethanol with a volume fraction of 75% for 1 min, then soaked in 20% (w / v) NaClO for 20 min, rinsed with sterile water for 3 to 4 times, blotted the surface moisture with sterile absorbent paper, and inoculated Cultivate on a hormone-free MS solid medium at 25°C under 16h / 8h (light / dark) light, and after 14 days, you can obtain an Artemisia annua sterile vaccine;

[0042] Step 2, according to the AaPDR1 gene promoter sequence in the whole genome of Artemisia annua, the promoter sequence was cloned by nested PCR;

[0043] In order to improve the specificity of the product, two rounds of nested PCR amplification were used, and the primers used are shown in Table 2. AaPDR1-FP1 and AaPDR1-RP1 were designed according to the AaPDR1 gene sequence and its ...

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Abstract

The invention relates to a promoter for regulating and controlling the expression of an AaPDR1 gene in glandular trichomes and application of the promoter. A nucleotide sequence of the promoter is shown as SEQ ID NO.1. Meanwhile, the invention also relates to a purpose of an AaPDR1 gene promoter in genetic engineering breeding for expressing and producing metabolites by plant glandular trichomes tissues. The promoter provided by the invention can guide a reporter gene to realize specific expression in plant glandular trichomes, and has important significance on the genetic engineering breeding for expressing and producing the metabolites by the plant glandular trichomes tissues.

Description

technical field [0001] The invention relates to a promoter and its application, in particular to a promoter for regulating gene expression in secretory glandular hairs and its use in the technical field of genetic engineering. Background technique [0002] Artemisia annua L. is an annual herb belonging to the family Asteraceae. Artemisinin, a sesquiterpene lactone oxide containing a peroxy bridge extracted from its aerial part, is currently the most widely used and effective antimalarial drug, especially for cerebral malaria and anti-chloroquine malaria. . Currently, artemisinin combination therapies (ACTs) are the most effective treatment for malaria recommended by the World Health Organization. However, the content of artemisinin in the plant Artemisia annua is low, which cannot fully meet the global market demand. Artemisia annua has glandular trichomes and nonglandular trichomes. There are a large number of secretory glandular hairs on the front and back of leaves, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/84A01H5/00
Inventor 唐克轩付雪晴石璞唐岳立沈乾孙小芬颜廷祥吕宗友
Owner 唐克轩
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