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Komagataeibacter nataicola and application thereof

A single colony technology of Gluconacetobacter, which is applied in the field of microbiology, can solve the problems of few reported strains, and achieve significant economic benefits, simple and easy separation and screening methods, and stable performance

Inactive Publication Date: 2015-12-09
南京荣之盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the isolated bacteria are all used in laboratory fermentation culture, and the strains used in large-scale industrial production are rarely reported

Method used

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  • Komagataeibacter nataicola and application thereof
  • Komagataeibacter nataicola and application thereof
  • Komagataeibacter nataicola and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] This embodiment relates to the isolation and screening of bacterial cellulose production strain - Gluconacetobacter RZS01, the strain is isolated from defective fruit, the specific steps are as follows:

[0027] After the defective fruit is crushed, immerse and shake it with sterile water for 20 minutes to make a 10-fold dilution, then draw the dilution, and transfer it into the enrichment culture medium at a volume ratio of 1:10 between the dilution solution and the enrichment medium. In a base test tube, it was cultured statically at 30°C for 7 days, and the liquid surface in the tube was selected to have a milky white bacterial cellulose film, and 1 g of the bacterial cellulose film was taken out by an aseptic method, ground in a sterile mortar, and then added to 9 mL of sterile normal saline to prepare into 10 -1 Bacterial suspension, then diluted step by step, spread on the separation plate medium, cultured at 30°C for 5 days, picked a single colony with good growt...

Embodiment 2

[0032] Production of bacterial cellulose by static culture:

[0033] Inoculate the strains into the seed medium first, shake and culture at 25°C for 12 hours, rotate at 100rpm, insert 2% of the inoculum into the fermentation medium, and culture at a constant temperature of 30°C for 2 days, then take out the bacterial cellulose membrane. After purification and weighing after drying, the yield of bacterial cellulose obtained was 3.56g / L.

[0034] Fermentation medium: glucose 22g / L, sucrose 12g / L, peptone 16g / L, disodium hydrogen phosphate 3.5g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.25g / L, acetic acid 2.4g / L, pH4 .2.

Embodiment 3

[0036] Production of bacterial cellulose by static culture:

[0037] First inoculate the strains into the seed medium, shake and culture at 35°C for 48 hours, with a rotation speed of 250rpm, insert 20% of the inoculum into the fermentation medium, culture at a constant temperature of 30°C for 10 days, and take out the bacterial cellulose membrane. After purification and weighing after drying, the yield of bacterial cellulose obtained was 3.42g / L.

[0038] Fermentation medium: fructose 25g / L, yeast extract powder 16g / L, sodium dihydrogen phosphate 2.9g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.25g / L, citric acid 0.4g / L, pH6.5 .

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Abstract

The invention discloses a komagataeibacter nataicola RZS01 strain which is preserved in China General Microbiological Culture Collection Center with a preservation numberof CGMCC No.10961. The bacterial strain is separated from defective fruits, and the separating and screening method is simple and convenient, so that the bacterial strain is easy to obtain. The bacterial strain is Gram-negative and short-bar-shaped aerobic bacteria, and is stable in performance. The komagataeibacter nataicola RZS01 is the bacterial cellulose producing bacteria, and can be used for producing bacterial cellulose through flask shaking fermentation, static state fermentation, fermentation tank large-scale fermentation or the combination of any two or three of the flask shaking fermentation, the static state fermentation and fermentation tank large-scale fermentation, the cellulose yield is high, large-scale industrial production is facilitated, and remarkable economic benefits are brought. The komagataeibacter nataicola RZS01 is nontoxic, free of pathogenicity, safe to people and livestock, and friendly to environment, serves as model organism for bacterial cellulose production, and has potential application prospects.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of Gluconacetobacter and its application. Background technique [0002] Cellulose is the most abundant natural polymer material in nature. Natural synthetic cellulose mainly comes from the photosynthetic synthesis of plants (ie plant cellulose) and microbial synthesis (ie microbial cellulose, also known as bacterial cellulose). [0003] Plant cellulose is widely used in industry, but its cellulose is doped with lignin and hemicellulose, which needs to be separated and purified before use. Bacterial cellulose has unique advantages in structure and properties, such as high purity, high tensile strength, good biocompatibility, and has a three-dimensional nano-network structure, and has good shape controllability, high Biocompatibility and good biodegradability. Based on the excellent properties of bacterial cellulose, commercialized products of bacterial cell...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04C12R1/02
Inventor 张衡朱春林杨加志自强孙汴京
Owner 南京荣之盛生物科技有限公司
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