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Hook-like trichoderma strain and application thereof

A technology of Trichoderma and strains, applied in the field of microorganisms, can solve the problems of chemical prevention and control of environmental pollution, unsafety, etc., and achieve the effect of simple and practical process, high control effect and stable control effect

Active Publication Date: 2015-12-09
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of Trichoderma hacinatum bacterial strain and its application in order to solve the environmental pollution and unsafe problems of chemical control in the prior art

Method used

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  • Hook-like trichoderma strain and application thereof
  • Hook-like trichoderma strain and application thereof
  • Hook-like trichoderma strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Isolation, purification and identification of Trichoderma:

[0027] Take soil samples evenly from the tomato planting fields in Shunyi, mix 10g of soil with 100ml of sterilized water respectively to make a suspension, and then carry out gradient dilution in turn, take 100 μl of the soil mixture diluted 10000 times and spread it on the PDA medium plate ( That is, 200 g of potatoes, 20 g of glucose, 17 g of agar, and distilled water to a volume of 1000 ml. This medium is sterilized at 121° C., and spread into a plate in a petri dish). PDA medium contains 75 μg / ml ampicillin, which inhibits bacterial growth. The culture medium was cultured at 25°C. After the tissue pieces grew colonies, single spores were isolated, and single colonies were picked and purified on PDA medium for classification and identification. The strains were morphologically determined according to the Fungal Identification Manual.

[0028] Pick the mycelium on the colony, put it into an EP tube filled...

Embodiment 2

[0033] Preparation and application of Trichoderma hacinata seed coating agent:

[0034] The Tham-21 strains were inoculated into 250ml Erlenmeyer flasks, each bottle containing 125ml of PDB liquid medium, and cultured at 25°C for 8 days with shaking (160rpm) to obtain a large number of mycelia and conidia. The liquid fermentation medium is a potato dextrose liquid (PDB) medium, that is, 200 g of potatoes, 20 g of glucose, and distilled water to a volume of 1000 ml.

[0035] The spore mycelium mixed solution obtained by liquid shaking culture (fermentation), the spore concentration is 10 7 pcs / ml), add the following ingredients: the ratio is as follows: 1L of spore liquid, 500g of diatomaceous earth, 200g of corn flour, and 50g of wheat bran. The solid fermented product is placed at room temperature and naturally dried for 3 days (the water content of the fermented product is controlled between 15-25%), crushed and passed through a 60-mesh sieve to obtain spore powder. The sp...

Embodiment 3

[0037] Preparation and application of Trichoderma hacinata seed coating agent:

[0038] The Tham-21 strain was inoculated into 250ml Erlenmeyer flasks, each bottle containing 125ml of PDB liquid medium, and cultured at 28°C for 6 days with shaking (160rpm) to obtain a large number of mycelium and conidia. The liquid fermentation medium is a potato dextrose liquid (PDB) medium, that is, 200 g of potatoes, 20 g of glucose, and distilled water to a volume of 1000 ml.

[0039] The spore mycelium mixed solution obtained by liquid shaking culture (fermentation), the spore concentration is 10 10pcs / ml), add the following ingredients: the ratio is as follows: 2L of spore liquid, 700g of diatomaceous earth, 400g of corn flour, and 150g of wheat bran. The solid fermented product is placed at room temperature to dry naturally for 5 days (the water content of the fermented product is controlled between 15-25%), crushed, and passed through a 60-mesh sieve to obtain spore powder. The spor...

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Abstract

The invention discloses a hook-like trichoderma strain and the application thereof. The strain is the hook-like trichoderma strain Tham-21 which is kept in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO.10923. The invention further discloses the application of a biocontrol seed coating prepared with the hook-like trichoderma strain Tham-21 in controlling root-knot nematodes. The hook-like trichoderma strain Tham-21 is a fungus separated from soil, is harmless to crops, can be used for controlling root-knot nematodes, is safe in use, and is suitable for biocontrol of root-knot nematodes. The second-stage root-knot nematode juvenile killing rate of a hook-like trichoderma strain Tham-21 fermentation broth is over 98%, the control effect in a greenhouse reaches 87.2%, the control effect is good and stable, and the requirement of agricultural production is met.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Trichoderma hacinata and its application. Background technique [0002] Root-knot nematode (Meloidogyne) is a highly specialized omnivorous plant pathogenic nematode. Nematodes known to harm vegetables mainly include root-knot nematode peanut, root-knot nematode northern, root-knot nematode southern, root-knot nematode java, and root-knot nematode beet. Nematodes have a wide range of hosts, often harming more than 30 kinds of vegetables such as melons, solanaceous fruits, beans, radishes, carrots, lettuce, and cabbage, and can also spread some fungal and bacterial diseases. Root-knot nematode is an important crop pathogen, causing losses in the order of billions of dollars each year. Root-knot nematodes mainly infect the roots of crops. After infection, the roots swell to form huge root knots, which can be connected to form beads. Because the roots are damage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/885
Inventor 茆振川马玉琴张晓平凌键陈国华杨宇红谢丙炎
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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