Method for purifying crude oil by using magnetic immobilized phosphatidase A2

Abstract

Prepared magnetic immobilized enzyme PLA2 is applied in the degumming process of soybean hydration degumming crude oil to improve the enzymatic hydrolysis efficiency of phosphatidase and reduce the content of free aliphatic acids in enzymatic hydrolyzed oil in order to solve the problems of difficult removal of non-hydratable phospholipids in the soybean crude oil purifying process, and high price, difficult recovery and rising of the content of the free aliphatic acids in oil of free phosphatidase. The optimum parameters of purification degumming of the soybean crude oil by the magnetic immobilized phosphatidase A2 are obtained through respectively researching influences of the magnetic immobilized enzyme addition amount, the reaction temperature, the reaction time and the reaction initial pH value on the phosphorus content as an index, and results show that the phosphorus content is 10.05mg / kg when the magnetic immobilized enzyme addition amount is 0.12g / kg oil, the reaction temperature is 55DEG C, the reaction time is 5h and the reaction initial pH value is 5.0, and the content of free aliphatic acids is 0.84g / 100g and is 0.14g / 100g lower than the content of free aliphatic acids in degummed oil obtained by using magnetic immobilized phosphatidase A1.

Description

Technical field: [0001] The invention relates to the field of oil processing technology, in particular to a magnetically immobilized phospholipase A 2 The method of refining crude oil. Background technique: [0002] Degumming is the first process of vegetable oil refining process, which mainly removes phospholipids and mucus substances. Phospholipids are mainly divided into non-hydratable phospholipids and hydrated phospholipids. The traditional hydration degumming method can remove hydratable phospholipids, and non-hydratable phospholipids with significant hydrophobicity need to use phospholipase to enzymatically hydrolyze non-hydrophilic substances to increase hydrophilicity and remove them from crude oil. Degumming is the removal of peptized impurities in oil. The main component is phospholipids. Among them, hydrated phospholipids can be removed by traditional hydration degumming, but non-hydratable phospholipids are difficult to remove due to their significant hydropho...

AI Technical Summary

Problems solved by technology

[0004] The present invention applies the prepared magnetically immobilized enzyme PLA2 in the degumming process of soybean hydration degumming crude oil to improve the enzymatic hydrolysis efficiency of phospholipase, reduce the free fatty acid content in the oil after enzymatic hydrolysis, and solve the problem of soybean crude oil refining process. Non-hydratable phospholipids are not easy to remove, and free phospholipases are expensive, difficult to recycle, and the content of free fatty acids in oils increases.