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Preparation method of grape seed extract

A technology for grape seeds and extracts, which is applied in the directions of solid solvent extraction, fat production, and fat oil/fat production, can solve the problems of high production cost, low extract purity, low yield and the like, and achieves the effect of improving yield

Active Publication Date: 2015-12-09
杭州再生肌生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the existing methods of extracting grape seed oil and proanthocyanidins from grape seeds include: aqueous solution immersion method, organic solvent extraction method, supercritical extraction method, etc., there are low yield, low purity of the extract, and production cost in these methods. high disadvantage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1) Pulverize the grape seeds, add 35% water and 0.1% yeast by weight of the grape seeds, seal and ferment at room temperature for 6 days, and vacuum-dry until the water content is ≤ 6%, to obtain the pretreated grape seed powder;

[0017] 2) Using the fluid composed of propane and dimethyl ether in a weight ratio of 2.5:6 as the extraction agent, the pretreated grape seed powder is subjected to subcritical extraction. The extraction temperature is 52.5°C, the extraction pressure is 2.7MPa, and the extraction time is 40min. The obtained extract is grape seed oil; after the grape seed oil is completely extracted, subcritical extraction is performed with absolute ethanol as the extractant, the extraction temperature is 60°C, the extraction pressure is 3.6MPa, the extraction time is 40min, and the extraction time is 40min. The product is concentrated under reduced pressure to 1 / 5 of the original volume, the concentrated solution is passed through an activated carbon column a...

Embodiment 2

[0019] 1) Pulverize the grape seeds, add 30% water and 0.1% yeast by weight of the grape seeds, seal and ferment at room temperature for 8 days, and vacuum-dry until the moisture content is ≤ 6%, to obtain the pretreated grape seed powder;

[0020] 2) The fluid composed of propane and dimethyl ether in a weight ratio of 2:5 is used as the extraction agent to carry out subcritical extraction on the pretreated grape seed powder. The extraction temperature is 53°C, the extraction pressure is 2.5MPa, and the extraction time is 50min. The obtained extract is grape seed oil; after the grape seed oil is completely extracted, subcritical extraction is performed with anhydrous ethanol as the extraction agent, the extraction temperature is 63°C, the extraction pressure is 3.4MPa, the extraction time is 40min, and the extraction time is 40min. The product is concentrated under reduced pressure to 1 / 6 of the original volume, the concentrated solution is passed through an activated carbon c...

Embodiment 3

[0022] 1) Pulverize the grape seeds, add 38% water and 0.15% yeast by weight of the grape seeds, seal and ferment at room temperature for 3 days, vacuum-dry until the moisture content is ≤ 6%, and obtain the pretreated grape seed powder;

[0023] 2) Using the fluid composed of propane and dimethyl ether in a weight ratio of 2.5:7 as the extraction agent, the pretreated grape seed powder is subjected to subcritical extraction. The extraction temperature is 49°C, the extraction pressure is 2.6MPa, and the extraction time is 60min. The obtained extract is grape seed oil; after the grape seed oil is completely extracted, subcritical extraction is performed with absolute ethanol as the extraction agent, the extraction temperature is 58 °C, the extraction pressure is 3.5 MPa, the extraction time is 50 min, and the extraction time is 50 min. The product is concentrated under reduced pressure to 1 / 3 of the original volume, the concentrated solution is passed through an activated carbon...

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Abstract

The invention discloses a preparation method of grape seed extract. The preparation method includes the steps that 1, grape seeds are smashed, water and yeast are added, fermentation is performed at indoor temperature in a sealed mode, and after vacuum drying, pretreated grape seed powder is obtained; 2, subcritical extraction is performed on the pretreated grape seed powder with fluid composed of propane and dimethyl ether as an extraction agent, extractive matter, namely, grape seed oil is obtained, subcritical extraction is performed with absolute ethyl alcohol as an extraction agent after complete extraction of the grape seed oil, vacuum concentration is performed on the extractive matter, and after concentrated liquor is dried through active carbon adsorption, red brown powder, namely, the procyanidine extract is obtained. Various effective extraction methods are combined in the extraction method, the yield can be effectively increased, the purity of the extract can be effectively improved, and then the product with extremely high quality can be obtained.

Description

technical field [0001] The invention relates to a preparation method of a plant extract, in particular to a preparation method of a grape seed extract. Background technique [0002] Grape seeds are mostly leftovers after grape wine making, which can account for 5% to 7% of the whole grape. Grape seeds are rich in grape seed oil and proanthocyanidins. Among them, the unsaturated fatty acid content in grape seed oil reaches 90%, and more than 75% of the unsaturated fatty acid is linoleic acid, which is a kind of vegetable oil with high quality. Proanthocyanidins have significant antioxidant and free radical scavenging activities, and have various functions such as cardiovascular protection, hypertension prevention, anti-mutation, anti-tumor, whitening and skin care, and have been widely used in food, medicine, cosmetics and other fields. And the existing methods of extracting grape seed oil and proanthocyanidins from grape seeds include: aqueous solution immersion method, or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62C11B1/04C11B1/02C11B1/10B01D11/02
CPCB01D11/02C07D311/62C11B1/025C11B1/04C11B1/10
Inventor 文继承
Owner 杭州再生肌生物科技有限公司
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