Preparation method and application of functional monascus-fermented corn bran food
A technology of functional food and corn husk, applied in the field of bioengineering, can solve the problems of not paying attention to the synthesis of functional substances of Monascus
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Embodiment 1
[0042] Embodiment 1 A kind of preparation method of red yeast corn husk functional food
[0043] The appearance of the red yeast corn husk functional food is pink to purple, with a unique fermented aroma, containing 10-50 mg / g of polysaccharides, 0.5-5 mg / g of lovastatin, and 0.4-6 mg / g of monascus pigment; The red koji corn husk functional food is prepared by using corn husk as the main raw material and Monascus as the strain, through test tube expansion culture, liquid shake flask culture, seed tank seed culture and solid fermentation culture.
Embodiment 2
[0044] Embodiment 2 A kind of preparation method of red yeast corn husk functional food
[0045] The method comprises the steps of:
[0046] a. Test tube expansion culture: inoculate Monascus strains in potato glucose medium, cultivate at a temperature of 20°C for 10 days, and store at 1°C to obtain Monascus test tube slant spores;
[0047] b. Liquid shake flask culture: Inoculate a ring of Monascus test tube slant spores into a 250mL Erlenmeyer flask filled with 20mL liquid shake flask culture medium. Cultivate for 18 hours to make liquid shake flask strains; wherein the weight of each component of the raw materials contained in each 1L medium is: bran 5g, glucose 5g, rice 1g, yeast extract 2g, magnesium sulfate 0.01g, dipotassium hydrogen phosphate 0.01g, the medium was sterilized at 100°C for 60 minutes.
[0048] c. First-level seed tank seed culture: Inoculate liquid shake flask strains into seed tank culture medium at an inoculum size of 1%, at a temperature of 20°C, a ...
Embodiment 3
[0050] Embodiment 3 A kind of preparation method of red yeast corn husk functional food
[0051] The method comprises the steps of:
[0052] a. Test tube expansion culture: inoculate Monascus strains in potato glucose medium, cultivate them at a temperature of 20°C for 5 days, and store them at 4°C to obtain Monascus test tube slant spores;
[0053] b. Liquid shake flask culture: Inoculate a ring of Monascus test tube slant spores into a 250mL Erlenmeyer flask filled with 20mL liquid shake flask culture medium. Cultivate for 40 hours to make liquid shake flask strains; wherein the weight of each component of the raw materials contained in each 1L medium is bran 5g, glucose 8g, rice 2g, yeast extract 3.5g, magnesium sulfate 0.05g, dipotassium hydrogen phosphate 0.05g, the medium was sterilized at 110°C for 50 minutes;
[0054] c. First-level seed tank seed culture: Inoculate liquid shake flask strains into the seed tank culture medium at an inoculum size of 1%, at a tempera...
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