Method for producing flavouring lotion by using saccharomycopsis fibuligera
A technology of capsule lamination and flavoring solution, which is applied in the biological field to achieve the effect of outstanding fragrance, not easy to pollute, and clear process
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Embodiment 1
[0019] A method for producing flavoring liquid by using the yeast Fumembranous saccharomyces, comprising the following steps:
[0020] (1) Strain activation: malt juice agar medium: malt extract powder 130g / L, chloramphenicol 0.1g / L, agar 15g / L, pH5.8-6.2. Inoculate the strain of yeast FBKL2.0094 on the slant medium, and cultivate it at 28°C for 48 hours, until the mycelium is full of the test tube;
[0021] (2) Preparation of seed liquid: Prepare liquid medium (malt extract powder 130g / L, chloramphenicol 0.1g / L, pH5.8-6.2), fill it in a triangular flask, sterilize at 115°C for 20min, and then put it on a clean bench Insert the activated strain into the liquid medium, and cultivate it on a shaker at 28°C for 48 hours to obtain a seed solution;
[0022] (3) Solid-state fermentation in a triangular flask: Sorghum wheat grain medium is used, wheat is crushed at 1:1, sorghum is crushed at 3:1, and then mixed with sorghum:wheat at a mass ratio of 1:1; then the grain is moistened w...
Embodiment 2
[0030] A method for producing flavoring liquid by using the yeast Fumembranous saccharomyces, comprising the following steps:
[0031] (1) Strain activation: malt juice agar medium: malt extract powder 130g / L, chloramphenicol 0.1g / L, agar 15g / L, pH5.8-6.2. Inoculate the strain of yeast FBKL2.0094 on the slant medium, and cultivate it at 28°C for 48 hours, until the mycelium is full of the test tube;
[0032] (2) Preparation of seed liquid: Prepare liquid medium (malt extract powder 130g / L, chloramphenicol 0.1g / L, pH5.8-6.2), fill it in a triangular flask, sterilize at 115°C for 20min, and then put it on a clean bench Insert the activated strain into the liquid medium, and cultivate it on a shaker at 28°C for 48 hours to obtain a seed solution;
[0033] (3) Solid-state fermentation in a triangular flask: Sorghum wheat grain medium is used, wheat is crushed at 1:1, sorghum is crushed at 3:1, and then mixed with sorghum:wheat at a mass ratio of 1:1; then the grain is moistened w...
Embodiment 3
[0041] A method for producing flavoring liquid by using the yeast Fumembranous saccharomyces, comprising the following steps:
[0042] (1) Strain activation: malt juice agar medium: malt extract powder 130g / L, chloramphenicol 0.1g / L, agar 15g / L, pH5.8-6.2. Inoculate the strain of yeast FBKL2.0094 on the slant medium, and cultivate it at 28°C for 48 hours, until the mycelium is full of the test tube;
[0043] (2) Preparation of seed liquid: Prepare liquid medium (malt extract powder 130g / L, chloramphenicol 0.1g / L, pH5.8-6.2), fill it in a triangular flask, sterilize at 115°C for 20min, and then put it on a clean bench Insert the activated strain into the liquid medium, and cultivate it on a shaker at 28°C for 48 hours to obtain a seed solution;
[0044] (3) Solid-state fermentation in a triangular flask: Sorghum wheat grain medium is used, wheat is crushed at 1:1, sorghum is crushed at 3:1, and then mixed with sorghum:wheat at a mass ratio of 1:1; then the grain is moistened w...
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