Preparation method and device of leukocyte-depleted platelet-rich plasma

A technology for platelet-rich plasma and leukocyte depletion, which is applied in separation methods, chemical instruments and methods, centrifuges, etc., can solve the problems of unsatisfactory PRP indicators, decreased efficacy, and insignificant clinical efficacy, and achieves good clinical application prospects. , The effect of short time-consuming and reducing the incidence of adverse reactions

Inactive Publication Date: 2017-09-22
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Abstract
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Problems solved by technology

[0003] There are three main indicators to evaluate the preparation method of P-PRP: first, the clearance rate of leukocytes, the higher the clearance rate, the more thorough the clearance of leukocytes, which can improve the clinical efficacy and reduce adverse reactions; secondly, the recovery rate of platelets, the higher the recovery rate, the blood The higher the degree of utilization; the second is the degree of platelet enrichment, only the onset dose of PRP is 4 to 6 times the degree of enrichment, too low degree of enrichment will lead to a decline in efficacy, resulting in insignificant clinical efficacy
However, due to the serious overlap between the distribution area of ​​leukocytes and the distribution area of ​​platelets, according to the current separation technology, the removal of more leukocytes will inevitably lead to the recovery of fewer platelets, so the leukocyte clearance rate and platelet recovery rate seem to be a contradiction.
[0004] At present, the Anitua method is mostly used to prepare PRP clinically, and there is also a method according to the Biomet company of the United States. The method provided by the platelet-rich plasma preparation kit prepares PRP, but the indicators of the prepared PRP are not ideal

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  • Preparation method and device of leukocyte-depleted platelet-rich plasma
  • Preparation method and device of leukocyte-depleted platelet-rich plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 PRP preparation experiment example (prepared by two conventional centrifuge tubes)

[0030] (1) Sample preparation:

[0031] Blood extraction: extract 4ml of anticoagulant and 36ml of whole blood respectively, add to the first centrifuge tube and mix well. In this example, ACD-A was selected as the anticoagulant.

[0032] (2) The first centrifugation:

[0033] For the first centrifuge tube, centrifuge at a centrifugal speed of 130g for 10 minutes to obtain a liquid divided into three layers, which are red, colorless and light yellow from bottom to top, wherein:

[0034] Red is the red blood cell layer, accounting for about 65% of the total volume, and its main component is red blood cells. In addition, it also contains a small amount of white blood cells and a very small amount of platelets, accounting for about 100% of the total number of red blood cells, 10-20% of the total number of white blood cells, and 100% of the total number of platelets. Less than ...

Embodiment 2

[0044] Example 2 PRP preparation experiment example (prepared by two conventional centrifuge tubes)

[0045] (1) Sample preparation:

[0046] Blood extraction: extract 4ml of anticoagulant and 36ml of whole blood respectively, add to the first centrifuge tube and mix well. In this example, ACD-A was selected as the anticoagulant.

[0047] (2) The first centrifugation:

[0048] For the first centrifuge tube, centrifuge at a centrifugal speed of 180g for 20 minutes to obtain a liquid divided into three layers, which are red, colorless and light yellow from bottom to top, wherein:

[0049] Red is the erythrocyte layer, accounting for about 40% of the total volume, and its main component is red blood cells. In addition, it also contains a small amount of white blood cells and a very small amount of platelets, accounting for about 100% of the total number of red blood cells, 10-20% of the total number of white blood cells, and 100% of the total number of platelets. Less than 10%...

Embodiment 3

[0059] Example 3 PRP Preparation Experimental Example (prepared by two conventional centrifuge tubes)

[0060] (1) Sample preparation:

[0061] Blood extraction: extract 4ml of anticoagulant and 36ml of whole blood respectively, add to the first centrifuge tube and mix well. In this example, ACD-A was selected as the anticoagulant.

[0062] (2) The first centrifugation:

[0063] For the first centrifuge tube, centrifuge at a centrifugal speed of 160g for 10 minutes to obtain a liquid divided into three layers, which are red, colorless and light yellow from bottom to top, wherein:

[0064] Red is the erythrocyte layer, accounting for about 45% of the total volume, and its main component is red blood cells. In addition, it also contains a small amount of white blood cells and a very small amount of platelets, accounting for about 100% of the total number of red blood cells, 10-20% of the total number of white blood cells, and 100% of the total number of platelets. Less than 1...

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Abstract

The invention belongs to the technical field of blood products, and discloses a preparation method of leukocyte-depleted platelet-rich plasma. Secondary centrifugation forms a centrifuge that is divided into three layers from bottom to top to obtain the upper platelet and plasma components; (iii) centrifuge the obtained upper layer again to form the bottom platelet deposition and plasma supernatant; extract part of the plasma supernatant, The remaining plasma supernatant was used to resuspend platelet deposits to obtain leukocyte-depleted platelet-rich plasma. The method realizes the elimination of white blood cells and the enrichment of platelets at the same time, and is convenient to operate and takes a short time, and the technical indicators of the prepared plasma products are all better than the existing similar products. The two centrifugations in the method provided by the invention can be carried out in two centrifuge tubes respectively, or in the same centrifuge device, and the invention also provides a centrifuge device for implementing the preparation method.

Description

technical field [0001] The invention belongs to the technical field of blood products, and in particular relates to a preparation method of blood plasma products and a centrifugal device for implementing the method. Background technique [0002] Platelet-rich plasma (PRP) is a blood product rich in platelets. According to whether it contains leukocytes, it can be roughly divided into two categories: L-PRP rich in leukocytes and P-PRP without leukocytes. In recent years, a large number of studies have confirmed that the active ingredient in PRP that promotes the repair and regeneration of bone tissue, cartilage tissue and tendon tissue is platelets, and the leukocytes in PRP not only do not promote tissue repair; The local immune response can inhibit tissue repair, thereby reducing the clinical efficacy of PRP and increasing the incidence of adverse reactions such as pain at the injection site. Therefore, the preparation method of leukocyte-depleted platelet-rich plasma is t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D17/038B04B5/00
Inventor 殷文靖张长青谢雪涛徐正良袁霆
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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