Paratyphoid c and salmonella cholerae identification kit and preparation and use methods thereof

Abstract

The invention relates to a paratyphoid c and salmonella cholerae identification kit and preparation and use methods thereof, and mainly solves the technical difficulty in quick screening and identification of paratyphoid c and salmonella cholerae in clinical infective cases and specimens such as an environment source, a food source and a livestock animal source. The paratyphoid c and salmonella cholerae identification kit comprises 1, salmonella C-group immune rabbit antiserum; 2, salmonella H-phase immune rabbit antiserum; 3, salmonella H-phase Vi immune rabbit antiserum; 4-6, dulcite, arabinose and tan sugar fermentation pipe; 7, mucic acid biochemical identification pipe; 8, potassium tartrate biochemical identification pipe; 9, sterilization mineral oil; 10, H-phase special induction soft agar; 11, induction serum H: c and H: 5 factor. The paratyphoid c and salmonella cholerae identification kit is mainly used for screening and identifying paratyphoid c and salmonella cholerae in conventional work.

Description

technical field [0001] The invention relates to the identification of Salmonella paratyphi C and Salmonella choleraesuis in the genus Salmonella, in particular to an identification kit for paratyphoid C and Salmonella choleraesuis, a preparation method and a use method. Background technique [0002] Salmonella is an important food-borne infectious pathogen, which widely exists in farmed animals and the environment. There are nearly 2,000 serotypes. Among them, most of the Salmonella of subgenus I that are related to human infection rely on serotype diagnosis, and some of them The antigenic formulas of the serotypes are the same. For example, 6,7:c:1,5 of group C correspond to 5 different serotypes: paratyphoid C, hog cholera, hog cholera Kongcheng astoria variant, hog cholera deguite variant, swine typhoid, especially type C Salmonella paratyphi 6,7:c(Vi):1,5 and cholera suis have very important clinical and veterinary diagnostic significance. However, the two have exactly...

AI Technical Summary

Problems solved by technology

However, the two have exactly the same O and H phase antigens, so it is difficult to distinguish them by serological methods

The "Law of the People's Republic of China on the Prevention and Control of Infectious Diseases" has different classifications of biological hazards and public health significance for the diseases caused by the two: Salmonella paratyphi C is a type B pathogenic bacterium that can cause typhoid-like cases and has important clinical and public health significance ;Salmonella choleraesuis is a Class C infectious disease, which is only seen in animal (little pig) epidemics and human bacteremia and deep infection cases, and there are no reports of foodborne outbreaks in humans, so its public health significance Relatively small

For a long time, clinical laboratories, public health and animal and veterinary laboratories have been unable to identify and accurately report the identification results due to the similarity of the two antigens, or the correct reporting time has been delayed due to the induction test of the antigen, which directly affects the national infectious disease Accurate case statistics of paratyphoid C and diagnosis and prevalence assessment of zoonotic diseases by reporting system

Many foreign professional laboratories usually use fluorescent PCR (RT-PCR) method to detect oriC Genes can be used to distinguish paratyphoid C from hog cholera, but this technology needs to rely on the equipment configuration of molecular diagnostic laboratories and reliable reference strains as controls, which is an obstacle to the popularization of most domestic grassroots laboratories