Xenopus laevis daudin skin antibacterial peptide and preparing method and application thereof

Abstract

The invention relates to xenopus laevis daudin skin antibacterial peptide and a preparing method and application thereof. The antibacterial peptide is separated from skin secretions of xenopus laevis daudin, it is obtained after purifying and sequencing are carried out that the sequence of the antibacterial peptide is Asp-Glu-Asp-Asp-Asp (DEDDD), and it is obtained through mass spectrometric that the molecular weight of the antibacterial peptide is 607.7 Da. According to the xenopus laevis daudin skin antibacterial peptide and the preparing method and application thereof, a physical method is adopted to stimulate the skin of xenopus laevis daudin, and thus the structure integrity and the biological activity of the antibacterial peptide are protected to a great degree. The antibacterial peptide has obvious bacteriostatic activity, low hemolytic activity and good breast cancer resisting activity. The molecular weight of the antibacterial peptide is low, so that the difficulty of chemosynthesis of the antibacterial peptide is lowered, and cost is greatly reduced. The antibacterial peptide has the obvious bacteriostatic action on gram-positive bacteria and gram-negative bacteria, is extremely low in hemolytic activity in the MIC value range, has high breast cancer resisting activity, can be used in various medical directions of preventing wound and trauma infection, resisting tumors, bacteriostatic preparations and the like, and has good application prospects.

Description

technical field [0001] The invention relates to the technical field of medicinal chemistry, in particular to a smooth Xenopus skin antibacterial peptide and its preparation method and application. Background technique [0002] Antibacterial peptides, also known as antimicrobial peptides, are substances with antimicrobial activity released by the immune system, which widely exist in organisms in nature as part of the innate immune components. Antimicrobial peptides are small molecule polypeptide substances with a wide range of biological activities, such as sterilization, anti-inflammatory, anti-virus, and tumor-inhibiting effects. They are an important part of biological non-specific defense systems. Antimicrobial peptides have broad-spectrum antibacterial activity, and can combine with lipopolysaccharide on the outer membrane of Gram-negative bacteria and peptidoglycan on the surface of Gram-positive bacteria, thereby destroying the cell membrane to form transmembrane chann...

AI Technical Summary

Problems solved by technology

The above two antimicrobial peptides are difficult and costly to synthesize due to their long sequences and secondary structures.

In the Chinese Patent Publication No. 103524602A, a six-peptide antibacterial peptide with the sequence RRRWWC is disclosed. However, this invention only clarifies its viral activity, and does not clarify its anti-tumor and other effects, and the patent does not study the antibacterial peptide. The hemolytic activity of the peptide greatly limits its application

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