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Method for extracting rice albumin and globulin mixed protein

An extraction method, a technology of protein extraction solution, applied in the direction of peptide preparation method, chemical equipment and method, peptide, etc., can solve the problem of difficult separation and extraction of albumin or globulin, and solve the problem of very low utilization rate, reduce loss, The effect of raising the level of research

Active Publication Date: 2015-11-25
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem that albumin or globulin in existing rice is difficult to separate and extract, and provides a method for extracting rice albumin and globulin mixed protein

Method used

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  • Method for extracting rice albumin and globulin mixed protein
  • Method for extracting rice albumin and globulin mixed protein
  • Method for extracting rice albumin and globulin mixed protein

Examples

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specific Embodiment approach 1

[0015] Specific embodiment one: the extraction method of rice albumin and globulin mixed protein of the present embodiment, carry out according to the following steps:

[0016] 1. Grinding rice into rice flour;

[0017] 2. Degreasing the rice flour with n-hexane for 3 to 5 times to obtain defatted rice flour;

[0018] Three, the defatted rice flour is obtained protein extract with salt solution;

[0019] Four, the protein extract is precipitated with trichloroacetic acid solution;

[0020] 5. Vacuum freeze-dry the precipitate to obtain the mixed protein of rice albumin and globulin.

[0021] This embodiment is improved on the basis of salt-soluble acid precipitation, extracting albumin that is difficult to separate or will greatly reduce the extraction rate of globulin after separation, and using trichloroacetic acid protein precipitation agent to directly precipitate the protein without adjusting the pH. Reduce operation steps and reduce losses. Through SDS-PAGE analysis,...

specific Embodiment approach 2

[0022] Embodiment 2: This embodiment is different from Embodiment 1 in that: the specific method of grinding rice into rice flour in step 1 is: grind fresh rice with a pulverizer for 10-15 seconds each time, 30-40 times in total. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0023] Specific embodiment three: what this embodiment is different from specific embodiment one is: in step 2, the concrete method that rice flour is degreased with n-hexane is:

[0024] A. Mix rice flour and n-hexane according to the ratio of 1g:4~6mL, stir at room temperature for 1h, let stand for 1h, and then centrifuge at 4000rpm for 20min to collect the precipitate;

[0025] B. Repeat step A for 2 to 4 times, and dry the collected precipitate in a water bath at 30° C. to obtain defatted rice flour. Others are the same as in the first embodiment.

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Abstract

The invention provides a method for extracting rice albumin and globulin mixed protein, relates to a method for extracting albumin and globulin mixed protein, and aims to solve the current problem that albumin or globulin in rice is difficult to separate and extract. The method comprises the following steps: 1, grinding rice into rice flour; 2, degreasing the rice flour with n-hexane for 3-5 times to obtain degreased rice flour; 3, preparing a protein extracting solution from the degreased rice flour by a salt dissolving method; 4, precipitating the protein from the protein extracting solution with a trichloroacetic acid solution; and 5, performing vacuum freeze-drying on the precipitate to obtain rice albumin and globulin mixed protein. The method can be used for extracting the rice albumin and globulin mixed protein simply, quickly and efficiently, and is applicable to industrial production; and the extraction rate of the extracted rice albumin and globulin mixed protein is over 97 percent, and the purity of the rice albumin and globulin mixed protein is over 90 percent. The method is used in the field of rice protein extraction.

Description

technical field [0001] The invention relates to a method for extracting albumin and globulin mixed protein. Background technique [0002] Rice is one of the most important food resources for human beings. Its protein is a low-allergenic protein recognized globally. It has a complete range of essential amino acids and high biological potency. Therefore, rice protein is considered as a plant protein resource with high development value, which has attracted the attention of researchers all over the world. However, compared with proteins from other grains, research results on rice protein are sparse. The reason is that unlike other grain proteins, almost all rice endosperm proteins are insoluble in water, salt solution and alcohol, which we call gluten. Therefore, most of the research records are always around the mixture of alkali-extracted rice proteins. There are even fewer studies on globulin and albumin, which are second only to glutenin, but there are many studies on glo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14
Inventor 杨林刘烨李慧王正旋梁明才
Owner HARBIN INST OF TECH
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