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Medicine for treating melanoma and preparation method of medicine

A melanoma and drug technology, applied in the field of medicine and biology, can solve the problems of affecting application, lack, and low absorption rate, and achieve the effect of low toxicity of normal human cells

Inactive Publication Date: 2015-11-25
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it has the following defects: low water solubility makes its absorption rate low; fast metabolism in the body leads to low bioavailability, thus affecting its clinical application
However, until now, due to the problem of drug resistance, there is still a lack of effective methods to treat this type of disease. Therefore, there is an urgent need to develop a new drug to overcome its drug resistance for the treatment of melanoma.

Method used

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  • Medicine for treating melanoma and preparation method of medicine
  • Medicine for treating melanoma and preparation method of medicine
  • Medicine for treating melanoma and preparation method of medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The D-borneol of the present embodiment (English name is naturalborneol, CAS accession number is 464-43-7, and molecular formula is C 10 h 17 OH, molecular weight is 154.24) concentration: 40 μ g / ml, curcumin concentration is 20 μ M.

[0026] 1. Anti-melanoma activity detection in vitro

[0027] (1) Experimental materials

[0028] The malignant melanoma cells A375 used in this experiment were purchased from ATCC in the United States; curcumin was purchased from Sigma; and dimethyl sulfoxide (DMSO), purchased from Sigma, USA; DMEM medium and trypsin, purchased from Invitrogen (Carlsbad, CA); 96-well plates, purchased from Corning, USA.

[0029] (2) Experimental method

[0030] A375 cells were cultured in DMEM medium containing 10% fetal bovine serum by volume. After the cells were cultured for 3 days, the growth status and cell density of the cells were observed. When the cells grew to 80%, the cells were subcultured. Transfer the cells digested with trypsin (conven...

Embodiment 2

[0062] The detection operation of embodiment 2 is basically the same as that of embodiment 1, except that the concentration of d-borneol in this embodiment is 40 μg / ml, and the concentration of curcumin is 80 μM.

[0063] The test results are as follows:

[0064] (1) Detection of anti-melanoma activity in vitro: the experimental results showed that, compared with Cur treatment alone, the cell survival rate decreased from 3.43±0.12% to 3.04±0.56% after combined treatment with NB and Cur. The experimental results showed that NB can increase curcumin (Cur) to inhibit the growth of A375 malignant melanoma cells.

[0065] (2) Detection of curcumin content in A375 cells: the experimental results showed that compared with the blank control group, the intracellular curcumin content of the NB group had no significant change after treatment, but the NB+Cur group significantly increased the intracellular Cur content and It is time dependent. The experimental results (Table 1) showed th...

Embodiment 3

[0078] The detection operation of embodiment 3 is basically the same as that of embodiment 1, except that the concentration of d-borneol in this embodiment is 20 μg / ml, and the concentration of curcumin is 40 μM.

[0079] (1) Detection of anti-melanoma activity in vitro: the experimental results showed that, compared with Cur treatment alone, the cell survival rate decreased after combined treatment with NB and Cur, from 21.49±1.60% to 19.85±0.54%, indicating that NB can improve Curcumin (Cur) inhibits the growth of A375 malignant melanoma cells.

[0080] (2) Detection of curcumin content in A375 cells: the experimental results (Table 5) showed that compared with the blank control group, the curcumin content in the cells of the NB group did not change significantly after treatment, but the NB+Cur group significantly increased the curcumin content in the cells. The content of Cur is time-dependent. Experimental results show that d-borneol significantly improves the absorption ...

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Abstract

The invention discloses a medicine for treating melanoma and a preparation method of the medicine. The medicine is prepared from active components or the active components and a carrier, wherein the active components are curcumin and d-borneol. The medicine can be prepared into required dosage forms; the d-borneol, the curcumin and the carrier are mixed according to different dosage forms; the medicine is prepared according to the preparation methods of different dosage forms; and when the dosage form is a slow-release tablet, the preparation principle refers to firstly preparation of the d-borneol and then release of the curcumin. The medicine is capable of effectively treating malignant melanoma; and the d-borneol and the curcumin are used in combination, so that the medicine is low in toxicity on normal cells of a human body, and is free of an obvious effect basically.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to a medicine for treating melanoma and a preparation method thereof. Background technique [0002] Curcumin (Curcumin, referred to as Cur), the chemical name is (E,E)-1,7-bis(4-hydroxy-5-methoxy)phenyl-1,6-heptadiene-3,5-di Ketones with the molecular formula C 21 h 20 o 6 , with a molecular weight of 368.37 g / mol. [0003] Curcumin is an active ingredient extracted from the root and stem of turmeric, which is widely used in food and medicine. It is often used as a spice, colorant, and food antioxidant in food, and as a potential hypoglycemic and antitumor drug in the field of medicine. Its appearance is orange-yellow powder, and its physical properties show a melting point of 183°C. It is hardly soluble in water and ether, but easily soluble in organic solvents such as ethanol, dimethyl sulfoxide (DMSO) and acetone. Studies have shown that curcumin ha...

Claims

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Application Information

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IPC IPC(8): A61K31/12A61P35/00A61K31/045
Inventor 苏健裕陈建平梅国栋李琳覃业霞
Owner SOUTH CHINA UNIV OF TECH
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