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A kind of culture medium of blueberry Mayden primary culture and its preparation method and application

A technology for primary culture and culture medium, which is applied to the culture medium and preparation field of the primary culture of blueberry mayden, and achieves the effects of robust growth, simplified culture process, and increased germination number.

Active Publication Date: 2017-03-29
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the tissue culture rapid propagation medium formula of Meideng is still blank at present.

Method used

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  • A kind of culture medium of blueberry Mayden primary culture and its preparation method and application
  • A kind of culture medium of blueberry Mayden primary culture and its preparation method and application
  • A kind of culture medium of blueberry Mayden primary culture and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 A kind of culture medium of blueberry Mayden primary culture

[0025] Improved WPM medium 12.724%, zeatin 0.01%, indole butyric acid 0.002%, glucose 74.79%, phytogel 12.474%. The improved WPM medium includes macroelements, microelements, iron salts, calcium salts and organic matter according to mass percentage. Among them, macroelements accounted for 74.9% of the mass of all WPM medium, including KNO 3 Potassium nitrate 1000mg / L, (NH4) 2 SO 4 Ammonium sulfate 300mg / L, MgSO 4 ·7H 2 O magnesium sulfate heptahydrate 400mg / L, KH 2 PO 4Potassium dihydrogen phosphate 200mg / L; trace elements account for 1.27% of the total WPM medium quality, including KI potassium iodide 1.1mg / L, MnSO4.4H2O manganese sulfate tetrahydrate 20mg / L, ZnSO4.7H2O zinc sulfate heptahydrate 8mg / L, H3BO3 boric acid 5mg / L; iron salt accounts for 0.35% of the mass of the entire WPM medium, Fe-EDDHA ethylenediamine o-diglycolic acid iron 10mg / L; calcium salt accounts for 19.71% of the m...

Embodiment 2

[0026] Embodiment 2: a kind of culture medium of blueberry Mayden primary culture

[0027] Improved WPM medium 12.724%, zeatin 0.01%, indole butyric acid 0.002%, glucose 74.79%, phytogel 12.474%. Among them, macroelements accounted for 74.1% of the mass of all WPM medium, including KNO 3 Potassium nitrate 900mg / L, (NH4) 2 SO 4 Ammonium sulfate 270mg / L, MgSO 4 ·7H 2 O magnesium sulfate heptahydrate 360mg / L, KH 2 PO 4 Potassium dihydrogen phosphate 180mg / L; trace elements account for 1.39% of the total WPM medium quality, including KI potassium iodide 1.0mg / L, MnSO4.4H2O manganese sulfate tetrahydrate 18mg / L, ZnSO4.7H2O zinc sulfate heptahydrate 6mg / L, H3BO3 boric acid 4mg / L; iron salt accounts for 0.425% of the total WPM medium mass, Fe-EDDHA ethylenediamine o-diglycolic acid iron 8mg / L; calcium salt accounts for 19.5% of the total WPM medium mass, CaCl2.2H2O dihydrate Calcium chloride 450mg / L; organic matter accounts for 4.585% of the total WPM medium mass, organic matt...

Embodiment 3

[0028] Embodiment 3: A kind of culture medium of blueberry Mayden primary culture

[0029] Improved WPM medium 12.724%, zeatin 0.01%, indole butyric acid 0.002%, glucose 74.79%, phytogel 12.474%. Among them, macroelements accounted for 74.1% of the mass of all WPM medium, including KNO 3 Potassium nitrate 1000mg / L, (NH4) 2 SO 4 Ammonium sulfate 300mg / L, MgSO 4 ·7H 2 O magnesium sulfate heptahydrate 400mg / L, KH 2 PO 4 Potassium dihydrogen phosphate 200mg / L; trace elements account for 1.27% of the total WPM medium quality, including KI potassium iodide 1.1mg / L, MnSO4.4H2O manganese sulfate tetrahydrate 20mg / L, ZnSO4.7H2O zinc sulfate heptahydrate 8mg / L, H3BO3 boric acid 5mg / L; iron salt accounts for 0.35% of the mass of the entire WPM medium, Fe-EDDHA ethylenediamine o-diglycolic acid iron 10mg / L; calcium salt accounts for 19.71% of the mass of the entire WPM medium, CaCl2.2H2O dihydrate Calcium chloride 500mg / L; organic matter accounts for 4.57% of the total WPM medium m...

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PUM

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Abstract

The invention discloses a Blomidon blueberry primary culture medium. The Blomidon blueberry primary culture medium is composed of, by mass, 12.724% of improved WPM media, 0.01% of zeatin, 0.002% of indolebutyric acid, 74.79% of glucose and 12.474% of phytagel. The invention further discloses a preparation method and application of the culture medium. Hormones in the culture medium are reasonable in matching, the primary culture and rooting culture are combined into one step, stems can root in the primary culture process, the culture process is simplified, and culture time is shortened. Due to the fact that the primary culture medium is used, the sprouting number of primary culture plants is increased, the plants grow strongly, and next-step subculture multiplication is facilitated.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, and in particular relates to a culture medium for primary culture of blueberry medden, a preparation method and application thereof. Background technique [0002] In the process of blueberry tissue culture rapid propagation, the most important step is the first step, the primary culture. The success of the primary culture directly determines the rapid propagation of the later tissue culture seedlings. And the culture medium formula is very important in the first generation culture, directly related to the survival rate that affects the first generation seedlings and the reproduction coefficient of the blueberry plant and the speed of reproduction. At present, for different blueberry varieties, there are many kinds of media for the primary culture that have been reported. But the formula of the culture medium that has been published at present is all aimed at different varieties, and not ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王媛花史红林颜志明王全智
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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