Tissue culture and rapid propagation technology of white poplar Gedayang having raised parts on branches and trunk

Abstract

The invention discloses a tissue culture and rapid propagation technology of white poplar Gedayang having raised parts on branches and trunk. The technology comprises explant preparation, induction, proliferation, rooting, rejuvenation, seedling hardening and transplanting. Tissue culture conditions comprise pH value of 5.4-5.8, a temperature of 25-28 DEG C, and natural illumination of 12h / d. An induction medium has a composition of MS+6BA(0.2 mg / L)+NAA(0.01 mg / L). A proliferation medium has a composition of MS+6BA(0.2 mg / L)+NAA(0.01-0.03 mg / L). A rejuvenation medium has a composition of 1 / 2MS+6BA(0.1 mg / L)+NAA(0.01 mg / L). A rooting medium has a composition of 1 / 2MS+IBA(0.1-0.2 mg / L). An induction rate is 98%. A multiplication coefficient is 4 or more. A 15d rooting rate is 80% or more. The number of produced roots is 2-4. The roots grow well. A transplant survival rate is 95% or more.

Description

technical field [0001] The invention relates to a tree tissue culture technology, in particular to a tissue culture propagation technology of endangered poplar pimples. Background technique [0002] Populus pimples, a variant of Populus alba, is named for its trunk and large branches that have ring-shaped or irregular protrusions, resembling pimples. Its branches are sparse, the crown is loose, the branches are sharp, and the skin is rough. Its wood texture is criss-crossed, showing natural and disordered various peculiar and beautiful patterns on the longitudinal section. It is an extremely rare special craft timber species. It has a wide range of uses, and its log resistance to vertical and horizontal pressure is much higher than that of ordinary poplar species such as arrow shaft poplar and Xinjiang poplar. It can be used as wooden bowls, wooden basins and other utensils, wooden furniture, and indoor carving artworks. It is also an excellent green ornamental tree specie...

AI Technical Summary

Problems solved by technology

The provenance of Populus pimples is scarce, and the cutting propagation materials are limited. The traditional forest tree propagation methods cannot solve the problem of its propagation protection, and rapid propagation is required.

[0004] The article "Tissue Culture Rapid Propagation Technology and Seedling Management of Populus pimples" published by Hu Shoujun et al. (Xinjiang Forestry, 2010, 5) provides a technical method for rapid propagation of Populus pimples by tissue culture, which can realize tissue culture and propagation of Populus pimples, but due to The selection of medium and hormone ratio and the conditions and methods of culture control are not ideal, the induced seedling rate is low, the reproduction coefficient and rooting coefficient are not high, and the survival rate of transplanting is not high, which affects the effect of tissue culture