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Triterpenoid saponin-type compounds of shinyleaf yellowhorn, as well as preparation method and application of compounds

A technology of triterpene saponins and compounds, applied in the field of medicine, to achieve a good effect of inhibiting activity

Inactive Publication Date: 2015-11-18
中国人民解放军第二一〇医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In the research on the chemical composition of X. sorbifolium, we found that its peel and stalk are rich in saponin, but its peel and stalk have not been developed and utilized as waste. In order to turn waste into treasure and improve the comprehensive utilization of X. sorbifolium value, we have systematically separated the chemical components of Xanthos sorbifolium husk and stalk, and systematically screened and investigated the activities of the obtained extracts and compounds

Method used

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  • Triterpenoid saponin-type compounds of shinyleaf yellowhorn, as well as preparation method and application of compounds
  • Triterpenoid saponin-type compounds of shinyleaf yellowhorn, as well as preparation method and application of compounds
  • Triterpenoid saponin-type compounds of shinyleaf yellowhorn, as well as preparation method and application of compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 The preparation method of Xanthos sorbifolia husk and fruit stalk extract 1.

[0056] Take 7kg of dried sorbifolia husk and stalk (collected in Chifeng City, Inner Mongolia in 2011), crush them, then add 70% ethanol according to the weight-to-volume ratio of 1:8, and reflux extraction for 3 times, each time for 2h. The resulting extract was concentrated by recovering the solvent under reduced pressure. The concentrated solution was extracted three times with equal volumes of ethyl acetate and n-butanol successively to obtain n-butanol extracts from the husk and peduncle of the fruit, and the solvent was removed, and the obtained brown extract was Extract 1 (350 g).

Embodiment 2

[0057] Embodiment 2: the preparation method of sorbifolia sorbifolium husk and fruit stalk extract 2.

[0058] Take 7kg of dried sorbifolia husk and stalk (collected in Chifeng City, Inner Mongolia in 2011), crush them, then add 70% ethanol according to the weight-to-volume ratio of 1:8, and reflux extraction for 3 times, each time for 2h. The resulting extract was concentrated by recovering the solvent under reduced pressure. The concentrated solution was separated and purified by macroporous resin (AB-8), and ethanol-water was used as solvent for gradient elution, and the 30%-80% ethanol extract was collected, and the obtained light yellow powder was the extract 2 (250g).

Embodiment 3

[0059] Example 3: Isolation of oleanene pentacyclic triterpene saponins from sorbifolia husk and stalk.

[0060] Get 9 kg of dry Xanthos sorbifolia husk and stalk (collected in Chifeng, Inner Mongolia in September, 2011), according to the preparation method of the extract in Example 1, reflux extraction with 70% ethanol for 3 times, the ratio of solid to liquid is 1:8, every After 2h, the solvent was recovered under reduced pressure and concentrated to 10L. Extract 3 times with equal volumes of ethyl acetate and n-butanol respectively to obtain 90 g of ethyl acetate extract and 450 g of n-butanol extract.

[0061] Get n-butanol extract 450g, carry out gradient elution with silica gel open column chromatography with dichloromethane:methanol:water (50:1:1-1:1:1) as the mobile phase, and the resulting fractions are subjected to silica gel thin-layer chromatography Fractions 1-5 were pooled as a guide for identical fractions.

[0062] Take Fraction 1 (dichloromethane: methanol: ...

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Abstract

The invention belongs to the technical field of medicine, discloses 13 types of novel oleanane-type pentacyclic triterpenoid-type compounds, six types of structures comprising the oleanane-type pentacyclic triterpenoid compounds, and preparation methods, and further provides extraction methods for extractives of shells and carpopodium of shinyleaf yellowhorn, and application of the compounds and the extractives to prevention of human cerebral glioma, human liver cancer, human pancreatic cancer, human stomach cancer and human colorectal cancer. The compounds and the extractives, which have antitumor activity, can be extracted from the shells and the carpopodium of shinyleaf yellowhorn, other plants of Sapindaceae, or other biological resources, and can also be obtained according to chemosynthesis.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a class of new saponin compounds in radix sorbifolia, a preparation method and application in the field of anti-human glioma, human colon cancer, human liver cancer, human pancreatic cancer and human gastric cancer. Background technique [0002] Xanthocerassorbifolia Bunge is a plant of the genus Xanthocerassorbifolia in the family Sapindaceae. Distributed in the hills and slopes of Northeast my country, North China, Shaanxi, Gansu, Ningxia, Anhui, Henan and other places. According to research, the saponins isolated from the husk, stalk, flower and other parts of X. sorbifolium not only have good anti-tumor activity, but also can significantly improve the learning ability of various memory impairment model mice, and improve the brain's hypoxia. Tolerance, prevention and treatment of memory retention disorders caused by multiple embolisms, reduction of the number of PC12 cell deat...

Claims

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Application Information

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IPC IPC(8): C07J63/00A61K31/704A61K36/77A61P35/00
CPCC07H1/08C07H15/256C07J63/008
Inventor 王晓波高慧媛王达袭荣刚赵林吴立军
Owner 中国人民解放军第二一〇医院
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