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Selective medium for multiplex proliferation of salmonella, listeria monocytogenes and vibrio parahaemolyticus and preparation method of selective medium

A technology for Listeria monocytogenes, Salmonella monocytogenes, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of inability of target bacteria to grow, inconsistent test results, loss of physiological characteristics, etc., and achieve a small inhibitory effect. , good bacteria-enhancing effect, good effect

Active Publication Date: 2015-11-11
舟山出入境检验检疫局综合技术服务中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the selective co-enrichment technology of Salmonella, Vibrio parahaemolyticus and Listeria monocytogenes
[0004] In addition, chilled aquatic products will be subjected to various damages during fishing, processing, and storage, which will make the bacteria in a sub-lethal state, which is prone to false negatives and missed detection
Although the bacteria in the sublethal state have certain activity, they lose some of the physiological characteristics of the normal bacteria, which will cause the selective substances in the selective medium to inhibit the non-target bacteria when the compound enrichment culture is carried out. The target bacteria in the sub-lethal state are also inhibited, so that the target bacteria in the sub-lethal state cannot grow, resulting in inconsistent detection results with the actual

Method used

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  • Selective medium for multiplex proliferation of salmonella, listeria monocytogenes and vibrio parahaemolyticus and preparation method of selective medium
  • Selective medium for multiplex proliferation of salmonella, listeria monocytogenes and vibrio parahaemolyticus and preparation method of selective medium
  • Selective medium for multiplex proliferation of salmonella, listeria monocytogenes and vibrio parahaemolyticus and preparation method of selective medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of selective medium for compound enrichment of Salmonella, Listeria monocytogenes and Vibrio parahaemolyticus:

[0029] Preparation of potassium tellurite solution: Weigh 0.00005 parts of potassium tellurite and add it into 10 parts of sterilized distilled water to obtain potassium tellurite solution and set it aside.

[0030] Preparation of acridine yellow solution: Weigh 0.1 part of acridine yellow and add it to 10 parts of sterilized distilled water to obtain acridine yellow solution and set it aside.

[0031] Preparation of nalidixic acid solution: Weigh 0.01 part of nalidixic acid and add to 10 parts of sodium hydroxide solution with a concentration of 0.05 mol / L to obtain nalidixic acid solution and set it aside.

[0032]Weigh 17 parts of tryptone, 3 parts of peptone, 10 parts of sodium chloride, 2.5 parts of sodium dihydrogen phosphate, 2.5 parts of glucose, 2.5 parts of mannitol, 0.02 parts of escin, 1 part of sodium citrate, 5 parts of skimmed milk ...

Embodiment 2

[0035] Preparation of selective medium for compound enrichment of Salmonella, Listeria monocytogenes and Vibrio parahaemolyticus:

[0036] Preparation of potassium tellurite solution: Weigh 0.00005 parts of potassium tellurite and add it into 10 parts of sterilized distilled water to obtain potassium tellurite solution and set it aside.

[0037] Preparation of acridine yellow solution: Weigh 0.1 part of acridine yellow and add it to 10 parts of sterilized distilled water to obtain acridine yellow solution and set it aside.

[0038] Preparation of nalidixic acid solution: Weigh 0.01 part of nalidixic acid and add to 10 parts of sodium hydroxide solution with a concentration of 0.05 mol / L to obtain nalidixic acid solution and set it aside.

[0039] Weigh 17 parts of tryptone, 3 parts of peptone, 10 parts of sodium chloride, 2.5 parts of sodium dihydrogen phosphate, 2.5 parts of glucose, 2.5 parts of mannitol, 0.02 parts of escin, 1 part of sodium citrate, 5 parts of skimmed milk...

Embodiment 3

[0042] Preparation of potassium tellurite solution: Weigh 0.00005 parts of potassium tellurite and add it into 10 parts of sterilized distilled water to obtain potassium tellurite solution and set it aside.

[0043] Preparation of acridine yellow solution: Weigh 0.1 part of acridine yellow and add it to 10 parts of sterilized distilled water to obtain acridine yellow solution and set it aside.

[0044] Preparation of nalidixic acid solution: Weigh 0.01 part of nalidixic acid and add to 10 parts of sodium hydroxide solution with a concentration of 0.05mol / L to obtain the nalidixic acid solution and set aside;

[0045] Weigh 20 parts of tryptone, 4 parts of peptone, 8 parts of sodium chloride, 2 parts of sodium dihydrogen phosphate, 3 parts of glucose, 3 parts of mannitol, 0.003 parts of escin, 1.5 parts of sodium citrate, 4 parts of skimmed milk powder, Add to 1000 parts of sterilized distilled water successively, mix well, adjust the pH of the resulting mixture to 7.5 with 1mo...

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Abstract

The invention relates to the technical field of detection of pathogenic bacterium, and discloses a selective medium for multiplex proliferation of salmonella, listeria monocytogenes and vibrio parahaemolyticus. The selective medium is prepared from tryptone, peptone, sodium chloride, sodium dihydrogen phosphate, glucose, mannitol, esculin hydrate, sodium citrate, skim milk powder, sterile purified water, potassium tellurite solution, acriflavine solution and nalidixic acid solution. A preparation method of the selective medium comprises the following steps: preparation of potassium tellurite solution; preparation of acriflavine solution; preparation of nalidixic acid solution; preparation of a semi-finished product medium; addition of a sample; and addition of potassium tellurite solution, acriflavine solution and nalidixic acid solution in the medium. The selective medium can carry out multiplex proliferation on the target bacteria and inhibit non-target bacteria, is small in inhibiting effect on the target bacteria in the sub-lethal state so that the target bacteria in the sub-lethal state can realize effective proliferation. The preparation method of the selective medium is easy to operate and high in efficiency.

Description

technical field [0001] The invention relates to the technical field of detection of pathogenic bacteria, in particular to a selective culture medium for compound enrichment of Salmonella, Listeria monocytogenes and Vibrio parahaemolyticus and a preparation method thereof. Background technique [0002] Food safety is a major global public health problem, and food contamination and foodborne diseases are still prevalent in both developed and developing countries. Bacterial foodborne diseases in my country are mainly caused by Salmonella and Vibrio parahaemolyticus. Although the incidence of Listeria Monocytogenes is not high, its fatality rate is much higher than other common foodborne pathogens. As high-protein, low-fat "white meat", seafood is delicious and nutritious, and is deeply loved by consumers. However, it is easily spoiled by bacterial contamination, which affects product safety. Therefore, Salmonella, Vibrio parahaemolyticus and Listeria monocytogenes are routine...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/42C12R1/01C12R1/63
Inventor 胡兴娟沈飚徐君辉邵宏宏周秀锦张静杨赛军
Owner 舟山出入境检验检疫局综合技术服务中心
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