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A preparation method of artificial heterogeneous antibacterial skin

A heterogeneous and artificial technology, applied in medical science, prostheses, etc., to achieve low wound shrinkage, reduce immunogenicity, and facilitate growth

Active Publication Date: 2018-06-29
上海泽充生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention not only solves the problem of industrial waste disposal, but also solves the problem of the source of artificial skin

Method used

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  • A preparation method of artificial heterogeneous antibacterial skin
  • A preparation method of artificial heterogeneous antibacterial skin
  • A preparation method of artificial heterogeneous antibacterial skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method of artificial heterogeneous skin includes the following steps:

[0032] (1) Take 12-month-old pigs that are qualified for quarantine, open the cavity aseptically, take the thoracic aorta, rinse it with medical saline, and bluntly separate the intima with a thickness of 0.3~0.5mm, and wash it with medical saline. Prepare the intima of the pig's thoracic aorta;

[0033] (2) Place the porcine thoracic aorta vascular intima obtained in step (1) in a 1M sodium chloride aqueous solution and oscillate at a speed of 100r / min in a constant temperature shaker at 37°C. Ultrasonic oscillation (frequency 40KHz) 5min, rinse with medical saline after 24h;

[0034] (3) Place the porcine thoracic aorta vascular intima obtained in step (2) in an aqueous solution of sodium lauryl sulfate with a solubility of 10 mg / mL, and oscillate at a rotation speed of 100 r / min in a constant temperature shaker at 37°C , Ultrasonic vibration (frequency 40KHz) every 6h for 5min, rinse wit...

Embodiment 2

[0044] A preparation method of artificial heterogeneous skin includes the following steps:

[0045] (1) Take 18-month-old pigs that are qualified for quarantine, open the cavity aseptically, take the thoracic aorta, rinse it with medical saline, and bluntly separate the vascular intima with a thickness of 0.3~0.5mm, and wash it with medical saline. Prepare the intima of the pig's thoracic aorta;

[0046] (2) Place the porcine thoracic aorta vascular intima obtained in step (1) in a 1M sodium chloride aqueous solution, and oscillate at a speed of 150r / min in a constant temperature shaker at 37°C. Ultrasonic oscillation (frequency 40KHz) 5min, rinse with medical saline after 24h;

[0047] (3) Place the porcine thoracic aorta intima obtained in step (2) in an aqueous solution of sodium lauryl sulfate with a solubility of 10 mg / mL, and oscillate at a speed of 150 r / min in a constant temperature shaker at 37°C , Ultrasonic vibration (frequency 40KHz) every 6h for 5min, rinse with medica...

Embodiment 3

[0057] A preparation method of artificial heterogeneous skin includes the following steps:

[0058] (1) Take a pig that has passed the quarantine inspection at the age of 24 months, open the cavity aseptically, take the thoracic aorta, rinse it with medical saline, and bluntly separate the vascular intima with a thickness of 0.3~0.5mm, and wash it with medical saline. Prepare the intima of the pig's thoracic aorta;

[0059] (2) Place the porcine thoracic aorta vascular intima obtained in step (1) in a 1M sodium chloride aqueous solution, and oscillate at a speed of 120r / min in a constant temperature shaker at 37°C. Ultrasonic oscillation (frequency 40KHz) 5min, rinse with medical saline after 24h;

[0060] (3) Place the porcine thoracic aorta intima obtained in step (2) in an aqueous solution of sodium lauryl sulfate with a solubility of 10 mg / mL, and oscillate in a constant temperature shaker at 37°C at a rotation speed of 120 r / min , Ultrasonic vibration (frequency 40KHz) every 6...

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Abstract

The invention discloses a preparation method of artificial heterogeneous antibacterial skin. In the method, the intima of the blood vessel is obtained after processing the pig thoracic aorta, and then passes through 1M sodium chloride solution, 10 mg / mL sodium lauryl sulfate solution and 2.5 mg / mL trypsin solution, rinsed, soaked in nano-silver solution, and cut into a suitable size according to clinical needs to obtain the artificial heterogeneous antibacterial skin. The artificial skin of the invention has rich sources of raw materials, short production period, significant antibacterial activity, long storage time and low price, and has great potential for clinical application.

Description

Technical field [0001] The invention relates to the technical field of tissue engineering biomaterials, in particular to a method for preparing artificial xenogeneic antibacterial skin by using pig thoracic aorta as a raw material. Background technique [0002] Skin is an organ with a high-level tissue structure covering the surface of the human body. It has the functions of protecting the human body, preventing bacterial erosion and infection, regulating body temperature and excreting waste fluids. When the skin is traumatized, burned or otherwise damaged, it will cause the loss of tissue and body fluid. As a result, the body's water, electrolyte, and acid-base balance will be unbalanced, affecting the body's normal absorption and metabolic balance. In addition, wounds exposed to the air are highly susceptible to bacteria, which can cause shock or sepsis in severe cases. Therefore, skin repair must be resolved. [0003] The ideal artificial skin should have the following charact...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/60
Inventor 邓超付海田王秀秀陈敬华
Owner 上海泽充生物技术有限公司
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