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Cultivation method for high-temperature-resisting pleurotus pulmonarius

A technique of lung-shaped lateral ear and cultivation method, applied in the directions of cultivation, plant cultivation, mushroom cultivation and the like

Active Publication Date: 2015-11-11
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on cultivating high-temperature Pleurotus pleurotus with rice straw

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The high-temperature-resistant Pleurotus pleurotus cultivation method of the present invention generally includes: using the high-temperature Pleurotus pleurotus strain CCMSSC00499 as a material, adopting PDA medium to activate it as a mother seed, and using a wheat grain medium to make the original seed, and the wheat grain medium is 98% Wheat, 2% Calcium Carbonate. Finally, 80-90% straw, 9-18% bran and 1-2% calcium carbonate are used to make cultivars, and they are packed into cultivation bags; after routine inoculation, they are cultivated at 20-28°C for 25-35 days until the hyphae are all overgrown The cultivation bag is finally cultivated at a temperature of 28-32°C and a relative air humidity of 80%-95% for 7-10 days until harvested.

[0014] The concrete steps of this method are:

[0015] Production of the original seed: Pleurotus pulmonarius (Fr.) Quél, whose strain preservation number is CCMSSC00499, was activated on ordinary PDA medium for 7 days as the mothe...

Embodiment 2

[0020] Operation with reference to the overall scheme of Example 1.

[0021] Production of the original seed: Pleurotus pulmonarius (Fr.) Quél, whose strain preservation number is CCMSSC00499, was activated on ordinary PDA medium for 7 days as the mother seed. Then the mother seed was inoculated on 98% wheat and 2% calcium carbonate, and cultivated in the dark at 25°C for 25 days to make a cultivar, which was put into a cultivation bag for use.

[0022] Cultivation material production: cut the whole straw into straw sections of about 3 cm, mix well according to the ratio of 90% straw, 8% bran, and 2% calcium carbonate, with a water content of 65%, and sterilize under high temperature and high pressure for 2 hours.

[0023] Planting method: Cultivate the above-mentioned cultivars into cultivation bags filled with cultivation materials, adopt stacked cultivation, generally 4 layers, cultivate at 25°C and avoid light for 30 days.

[0024] Management and protection method: the te...

Embodiment 3

[0026] Operation with reference to the overall scheme of Example 1.

[0027] Production of the original seed: Pleurotus pulmonarius (Fr.) Quél, whose strain preservation number is CCMSSC00499, was activated on ordinary PDA medium for 7 days as the mother seed. Then the mother seed was inoculated on 98% wheat and 2% calcium carbonate, and cultivated in the dark at 25°C for 28 days to make a cultivar, which was put into a cultivation bag for use.

[0028] Cultivation material production: cut the whole straw into straw sections of about 3 cm, mix well according to the ratio of 85% straw, 14% bran, and 1% calcium carbonate, with a water content of 65%, and sterilize under high temperature and high pressure for 2 hours.

[0029] Planting method: Cultivate the above-mentioned cultivars into cultivation bags filled with cultivation materials, adopt stacked cultivation, generally 5 layers, and cultivate at 28°C in the dark for 28 days.

[0030] Management and protection method: Culti...

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PUM

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Abstract

The invention discloses a cultivation method for high-temperature-resisting pleurotus pulmonarius. The method comprises the steps of preparation of breeder seeds and mother strains, preparation of cultivated species, spawn running and fruiting management. The high-temperature-resisting pleurotus pulmonarius strains CCMSSC00499 in the national strain conservation center are taken as materials, activated by PDA culture media to be used as mother strains and made into breeder seeds by means of wheat grain culture media, and finally 80-90% of straw, 9-18% part of bran and 1-2% of calcium carbonate are adopted for manufacturing cultivation bags; after conventional inoculation, culture is carried out for 25-35 days at 20-28 DEG C, and then hyphae overgrow on the cultivation bags, and the pleurotus pulmonarius is harvested after culture is carried out for 7-10 days under the temperature being 28-32 DEG C and the relative air humidity being 80-95%. By means of the cultivation method for high-temperature-resisting pleurotus pulmonarius, a new high-temperature cultivation bacterial strain is provided for the edible mushroom industry, and genetic resources are enriched.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for cultivating edible fungus high temperature-resistant Pleurotus pleurotus, including the proportioning of culture materials and the management steps of fruiting and the like. Background technique [0002] Pleurotus pulmonarius (Fr.) Quél, the trade name is Pleurotus, which belongs to Basidiomycotina, Phytomycota, Agaricaceae, Pleurotaceae, and Pleurotus in taxonomy. It is the most important traditional cultivation in my country. One of the varieties, mainly distributed in Henan, Guangxi, Guangdong, Shaanxi, and Tibet. The lung-shaped Pleurotus not only has the characteristics of fast growth, high yield, delicious taste and rich nutrition, but also contains a variety of amino acids and vitamins, and its physiologically active substances can improve human immune function, prevent cancer, and fight cancer. At present, high-temperature lung-shaped side ear implant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00A01G18/20
Inventor 李静杨祝良刘晓斌颜晨
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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