A Micro-droplet Fusion Method Based on Hydrophilic Filament Induction

A technology of hydrophilic fiber filaments and fusion method, applied in the field of microfluidics, can solve the problems of influence and droplet stability, and achieve the effect of avoiding the production process and high-efficiency application potential.

Active Publication Date: 2016-11-23
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in such methods, surfactants are usually not used
However, surfactants play an important role in maintaining the stability of droplets, and its lack will lead to a decrease in the stability of droplets, which directly affects the subsequent manipulation of droplets such as sorting, capture, incubation, etc.

Method used

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  • A Micro-droplet Fusion Method Based on Hydrophilic Filament Induction
  • A Micro-droplet Fusion Method Based on Hydrophilic Filament Induction

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Experimental program
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Embodiment

[0020] The fusion of microdroplets induced by hydrophilic filaments is realized on a microfluidic chip. The specific method is as follows: the production of the microfluidic chip is to use the photoetching technology to make the SU-8 template first, then pour polydimethylsiloxane on the template, polymerize in an oven at 80°C for 2 hours, and print the microstructure The polymer is peeled from the mold. Then, the hydrophilic glass filaments were embedded in the microchannel, and the flat polydimethylsiloxane sheet was treated with oxygen plasma and sealed together. Place the sealed chip in an oven at 80°C overnight to restore the hydrophobicity of the polydimethylsiloxane.

[0021] The design of the chip includes three T-shaped structures arranged in parallel (see figure 1 ). Surfactant (2.5% Span 80) was blended into the oil phase (mineral oil). Driven by the external pump, the oil phase flow rate is 0.1 μL / min, and the water phase (ultrapure water) flow rate is 0.1 μL / mi...

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Abstract

The invention relates to a micro-fluidic technology, and particularly relates to a micro-droplet fusion method based on hydrophilic cellosilk induction. Specifically, hydrophilic cellosilk is embedded into a micro-channel of a chip, a surfactant is mixed into an oil phase, when micro-droplets on two wings of the chip flow through the hydrophilic cellosilk, the parts, contact with the hydrophilic cellosilk, of the micro-droplets can be extended and deformed, so that water-oil interfaces of the micro-droplets are unstable, and then the micro-droplet fusion is triggered. The micro-droplet fusion method provided by the invention is easy to operate, and can efficiently achieve stable droplet fusion of the surfactant without requiring the aid of any external energy sources.

Description

technical field [0001] The invention relates to microfluidic technology, in particular to a micro-droplet fusion method induced by hydrophilic fiber filaments. Background technique [0002] Micro-droplets are formed under the interaction of flow shear force and surface tension by taking advantage of the immiscibility of water and oil. Highly monodisperse microdroplets can be produced on a chip in a high-throughput and controllable manner using microfluidic technology. As an independent reactor, the microdroplet has the characteristics of small volume (picoliter to nanoliter), low risk of diffusion and cross-contamination between samples, and rapid mixing of inclusions. At present, micro-droplet technology has been widely used in the synthesis of new materials, cell culture and screening, protein crystallization, detection of biomarkers and other fields. [0003] For chemical and biological reaction systems, the amount and order of addition of reagents are directly related ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00B01F3/08
Inventor 李春宇马波徐健
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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