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Preparation method of disulfide dot/nanosheet compound DNA electrochemical probe

A disulfide and nanosheet technology, applied in the field of analytical chemistry, can solve the problems that transition metal disulfide quantum dots/nanosheet composite bioelectrochemical probes have not yet been seen, and achieves high sensitivity, low detection limit, good stability

Active Publication Date: 2015-10-28
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Up to now, transition metal disulfide quantum dots (especially titanium disulfide and tantalum disulfide) have been less studied in the field of biosensing. Bioelectrochemical probes have not yet been reported in domestic and foreign literature and related patents

Method used

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  • Preparation method of disulfide dot/nanosheet compound DNA electrochemical probe
  • Preparation method of disulfide dot/nanosheet compound DNA electrochemical probe
  • Preparation method of disulfide dot/nanosheet compound DNA electrochemical probe

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Weigh 200mg of molybdenum disulfide powder and disperse it in 10mL of organic solvent N-methylpyrrolidone. After ultrasonic stripping for 3 hours, let the dispersion stand for 10 minutes, collect most of the dispersion in the upper layer, and remove the large particles that are not completely peeled off at the bottom to obtain a single layer or several layers of molybdenum disulfide nanosheet dispersion. Stir the dispersion liquid vigorously at 135°C for 7 hours to obtain a composite solution of molybdenum disulfide quantum dots / nanosheets, add acetone to the above composite solution, and the composite aggregates to the top layer of the solution, separate and dry at room temperature to obtain Composite of molybdenum disulfide quantum dots / nanosheets. Take 100mg of the molybdenum disulfide quantum dot / nanosheet composite prepared above and dissolve it in 10mg·mL -1 In the mixed solution of thionine acetate and ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate, ...

Embodiment 2

[0036] Weigh 200mg of titanium disulfide powder and disperse it in 10mL organic solvent N,N-dimethylformamide. After ultrasonic stripping for 6 hours, let the dispersion stand for 10 minutes, collect most of the dispersion in the upper layer, and remove the large particles that are not completely peeled off at the bottom. , to obtain a single-layer or several-layer titanium disulfide nanosheet dispersion. Vigorously stir the dispersion at 125°C for 8 hours to obtain a composite solution of titanium disulfide quantum dots / nanosheets, add chloroform to the above composite solution, the composite gathers to the top layer of the solution, separate, and dry at room temperature to obtain Composite of titanium disulfide quantum dots / nanosheets. Take 200mg of the titanium disulfide quantum dot / nanosheet composite prepared above and dissolve it in 10mg·mL -1 In the mixed solution of thionine acetate and ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate, the above mixture is...

Embodiment 3

[0040] Weigh 200mg of tantalum disulfide powder and disperse it in 10mL organic solvent N,N-dimethylformamide. After ultrasonic stripping for 9 hours, let the dispersion stand for 10 minutes, collect most of the dispersion in the upper layer, and remove the large particles that are not completely peeled off at the bottom. , to obtain a single-layer or several-layer molybdenum disulfide nanosheet dispersion. The dispersion was stirred vigorously at 140°C for 6 hours to obtain a composite solution of tantalum disulfide quantum dots / nanosheets, centrifuged in a centrifuge at a speed of 8000rpm for 6 minutes, and dried at room temperature to obtain tantalum disulfide quantum dots / nanosheets compound. Take 100 mg of the tantalum disulfide quantum dot / nanosheet composite prepared above and dissolve it in 10 mg·mL -1 In the mixed solution of thionine acetate and ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate, the above mixture is ultrasonicated for 2 hours at room temp...

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Abstract

The invention relates to a preparation method of a disulfide dot / nanosheet compound DNA electrochemical probe. The method concretely comprises the following steps: 1, carrying out ultrasonic peeling on transition metal disulfide powder, carrying out high-temperature electromagnetic stirring to prepare a transition metal disulfide quantum dot / nanosheet compound, and carrying out ultrasonic treatment on the compound in a thionine and ionic liquid dispersion liquid to prepare a thionine functionalized compound; 2, dispersing the compound prepared in step 1 in an organic solvent to prepare a suspension, dispensing the suspension on an electrode, and drying to obtain a compound modified electrode; and 3, carrying out electrochemical test on a three-electrode system formed by the electrode produced in step 2 in a buffer solution, injecting different concentrations of a DNA solution, testing, recording the change of the electrochemical signal of the electrode, and constructing a DNA electrochemical probe system. The probe has the advantages of high sensitivity, good stability, wide linear range and low detection limit, can be used in detection of DNA in serum, and has wide application prospect in biological analysis and clinic diagnosis fields.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a preparation method of a disulfide dot / nano sheet composite DNA electrochemical probe. Background technique [0002] Deoxyribose nucleic acid (Deoxyribonucleic acid, abbreviated as DNA) is the basic genetic material of all organisms. DNA detection plays a vital role in the fields of medical diagnosis, bioengineering and environmental protection. As a new type of detection method, DNA electrochemical bioprobe has many advantages such as simple equipment required, fast and sensitive detection, and related technologies are developing very rapidly. In recent years, the development of nanoscience and technology is changing with each passing day, which provides a new and effective way for the development of new biological probes with high sensitivity, high stability, low cost and good safety. At present, DNA bioprobes based on nanomaterials such as gold nanop...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 王宗花赵春芹桂日军金辉杨敏夏延致
Owner QINGDAO UNIV
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