In-vitro biological valve calcification evaluation method and anti-calcification factor solution

Abstract

The invention relates to the technical field of biological valves, and provides an in-vitro biological valve calcification evaluation method. The evaluation method comprises the following steps: preparing a calcification evaluation solution; pre-heating a RCI solution; carrying out a PRIC treatment; preserving a sample; carrying out in-vitro calcification reactions; and detecting the calcium quantitatively. The anti-calcification factor solution comprises an alcohol reagent (A), an alkali solution (B), a reagent of grease and derivatives thereof (C), and a buffer solution (D). The provided evaluation method has the advantages of short evaluation period, reduced evaluation steps, and saved evaluation cost. At the same time, the anti-calcification factor solution is used to treat biological valve chemically to enclose some free chemical groups, thus the binding strength between biological valve and calcium ions is reduced, the calcium content of biological valve is reduced, and the anti-calcification performance of the biological valve is enhanced. The provided in-vitro biological valve calcification evaluation method can rapidly and effectively judge the biological valve anti-calcification performance after a chemical treatment.

Description

technical field [0001] The invention relates to the field of biological valves, in particular to a method for evaluating the calcification of biological valves in vitro and an anti-calcification factor solution. Background technique [0002] At present, among the elderly over 65 years old, the incidence of aortic valve stenosis due to aortic valve calcification is 2% to 7%, and the proportion of aortic valve stenosis increases with age. Patients with severe aortic stenosis have severely impaired left ventricular function, and the quality of life of the patients is reduced and the survival time is significantly shortened. Effective treatment is necessary. [0003] For severe aortic valve patients with surgical indications, surgical aortic valve replacement (whether a mechanical valve or a biological valve is used) is still the preferred treatment, and the aortic valve area after replacement is often greater than 1.5 cm 2 . However, for those patients with advanced age, end-...

AI Technical Summary

Problems solved by technology

[0005] The method of evaluating biological valve calcification through animal clinical practice has certain limitations. It has high requirements for clinical surgery and clinicians' technical dependence, and has corresponding requirements for animal selection. At the same time, the breeding cycle is long and affected by the feeding environment and individual differences. Large, other symptoms may appear, such as bioprosthetic epidermis, growth vegetation, etc., which cannot accurately and effectively reflect the chemical interaction between bioprosthesis and calcium ions after chemical treatment

Existing methods not only increase the technical difficulty, but also greatly increase the time and cost. At the same time, the evaluation of the calcification performance of the biological valve is affected by various factors.

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