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Culture method of bacillus amyloliquefaciens and application thereof

A technology of amylolytic bacillus and bacillus, applied in the field of microorganisms, can solve problems affecting human health, chemical residues, etc., and achieve the effect of simple use, low cost, and environmental friendliness

Inactive Publication Date: 2015-10-21
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] Aiming at the deficiencies of the prior art, the present invention provides a method for cultivating Bacillus amyloliquefaciens and its application, which can solve the problem of chemical residues produced by chemical agents for fresh fruits and vegetables, thereby affecting the health of the body

Method used

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  • Culture method of bacillus amyloliquefaciens and application thereof
  • Culture method of bacillus amyloliquefaciens and application thereof
  • Culture method of bacillus amyloliquefaciens and application thereof

Examples

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Embodiment

[0041] 1 Isolation and purification of antagonistic strains

[0042] The bee grain was cultured with beef extract peptone liquid medium and potato glucose liquid culture respectively based on 28-37°C for 24-48 hours, and the dilution plate method was used to separate microorganisms, and the bacteria and fungi were separated using beef extract peptone liquid medium ( NA) and potato dextrose liquid medium (PDA) plates, respectively placed in 30 ~ 37 ℃ and 25 ~ 30 ℃ incubator culture 2 ~ 3d. According to the characteristics of the colony's shape, color, transparency, etc., single colonies with obvious morphological differences were picked for streaking and purification, and the slopes were stored at 4°C for later use. A total of 56 bacterial strains were obtained.

[0043] 2 Screening of antagonistic strains

[0044] 2.1 Fermentation broth preparation

[0045]Inoculate the bacterial strain to be screened in potato glucose liquid medium, and ferment and culture in a shake flask...

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Abstract

The invention discloses a culture method of bacillus amyloliquefaciens and an application thereof and belongs to the technical field of microorganisms. The preservation number of the bacillus amyloliquefaciens is CCTCC No: M2015435. The culture method and the application have the advantages that bacillus amyloliquefaciens strain fermentation liquor has broad-spectrum bacteriostatic activity, sickle fruit rot germs generated after tomatoes are harvested can be effectively controlled, and a novel effective, non-toxic, non-residual and environment-friendly biological control way is provided for preservation and freshness after the tomatoes are harvested, reduction of postharvest loss and improvement of product quality and safety. A strain fermentation medium is simple in requirement and low in cost, the culture method is simple, a related preservative preparation process is simple, and an application method is simple and suitable for being applied and popularized in production.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for cultivating a strain of Bacillus amyloliquefaciens and its application. Background technique [0002] Fruits and vegetables are important non-staple foods in people's lives, the second largest category of agricultural products after grain, and important raw materials for the food industry. Tomato is an important vegetable variety. There are many kinds of tomato postharvest storage diseases. Among them, Fusarium tomato fruit rot is a postharvest pathogenic fungus discovered in recent years. It mainly infects mature fruits and causes fruit soft rot. greater economic loss. [0003] my country's fruit and vegetable storage and preservation technology is relatively backward compared with developed countries, and the main method is to spray chemically synthesized fungicides for fruit and vegetable preservation. Long-term use will not only produce drug ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23B7/155C12R1/07
CPCA23B7/155C12P1/04C12N1/205C12R2001/07
Inventor 申光辉冯孟张志清秦文陈安均刘书亮黎杉珊林德荣陈姝娟何利
Owner SICHUAN AGRI UNIV
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