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Full-automatic polypeptide extraction time-of-flight mass spectrometry detector

A mass spectrometer and spectral analysis technology, applied in the field of protein detection, can solve the problems of ineffective large-scale, time-consuming, and low throughput.

Active Publication Date: 2015-10-14
BIOYONG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has many shortcomings such as time-consuming, low efficiency, low throughput, poor consistency, and possible cross-contamination. High volume protein extraction and mass spectrometry requirements

Method used

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  • Full-automatic polypeptide extraction time-of-flight mass spectrometry detector
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  • Full-automatic polypeptide extraction time-of-flight mass spectrometry detector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment one: the extraction of protein sample in body fluid and mobile distribution process (see Figure 4-6 )

[0058] 1. Add reagents and samples

[0059] In a 96-type deep well plate, add sample-magnetic beads-binding solution, sample-magnetic beads-binding solution, sample-magnetic beads-binding solution, washing solution, washing solution, washing solution , washing solution, washing solution, washing solution, eluting solution, eluting solution, eluting solution, and seal the 96-type deep-well plate with parafilm and place it in a moderate environment. Wherein the magnetic beads, binding solution, washing solution and eluent can all use reagents in known magnetic beads-protein extraction kits.

[0060] The sample plate to which the reagent has been added is fixed on the sample plate fixing table 110 according to the correct orientation.

[0061] 2. Setting method and parameters

[0062] Use an external computer to set up the method, take an operation name, ...

Embodiment 2

[0067] Embodiment two: sample packing

[0068] 1. Preparation

[0069] Put the sample to be divided into the sample collecting plate 206, install it on the sample plate fixing table 110, and move to the position of the right locking slot 113 driven by the stepping motor 112. Fix the sample collection plate 206 in the corresponding position.

[0070] 2. Method parameter setting

[0071] In the external computer program, take an operation name, such as "sample dispensing", and then set the well position of the required dispensing sample and the corresponding well position in the sample collection plate, dispensing volume, pipetting speed and other parameters. After setting all the parameters, save the method and select the method in the operation.

[0072] 3. Packing samples

[0073] Select the corresponding method and start dispensing samples. Under the control of the external computer, the pipette 204 moves to the top of the cleaning device 32, and is successively cleaned...

Embodiment 3

[0074] Embodiment three: Chip sample spotting (referring to Figure 7-9 )

[0075] Under the control of an external computer program, the sample pointing device first cleans the sample point needle 306 . The spotting instrument 3 or the spotting device 3 moves to the top of the cleaning device 32, successively washes through 100% alcohol, 50% alcohol and ultrapure water, then dries in the drying chamber 325, and then moves to the top of the sample collection plate 206 , accurately absorb the extraction solution and its volume, then move to the top of the chip 307, and accurately point the extraction solution on the set target position. Complete a spotting cycle. In this way, all the extracts that need to be spotted are spotted on the corresponding target positions. Let it dry naturally at room temperature, and then apply the matrix.

[0076] The prepared matrix solution is added to the matrix plate, the sample collection plate 206 is removed, and the matrix plate is fixed ...

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Abstract

The present invention provides a full-automatic polypeptide extraction time-of-flight mass spectrometry detector capable of concurrently extracting bulk samples. The full-automatic polypeptide extraction time-of-flight mass spectrometry detector comprises a polypeptide extraction and purification device, a pipetting distributing device, an automatic sample adding device and a mass spectrometer. According to the present invention, the electromagnetic rod and magnet rod sleeve mode is used so as to completely separate the sample; the polypeptide chip is prepared through the automatic sample adding device; the polypeptide mass spectrum map is obtained through the laser sampling of the mass spectrometer; and the full-automatic polypeptide extraction time-of-flight mass spectrometry detector has characteristics of less time consumption, high efficiency, good uniformity and sample cross-contamination avoiding, and can achieve the same technical effect as the manual operation effect of the existing mass spectrometer.

Description

technical field [0001] The present invention relates to an automatic system for body fluid protein extraction and mass spectrometry spotting. It combines the magnetic bead method to extract protein in body fluid to realize the automatic operation of mass spectrometer chip spotting, so as to prepare biochips, and obtain protein by laser sampling of mass spectrometers. The mass spectrogram and related information can be used to prepare biochips, so it belongs to the field of protein detection. Background technique [0002] With the implementation and advancement of the Human Genome Project, life science research has entered the post-genome era. In this era, the main research object of life science is functional genomics, including structural genome research and proteome research. Although the genomes of several species have now been sequenced, the functions of more than half of the genes in these genomes are usually unknown. At present, the strategies adopted in functional g...

Claims

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Application Information

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IPC IPC(8): G01N27/62G01N1/28
Inventor 马庆伟林燕
Owner BIOYONG TECH
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