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Polypeptide CJ128 resistant to HIV-1 activity and application of polypeptide CJ128

A technology for HIV-1 and HIV-1B, which is applied to the anti-HIV-1 active polypeptide CJ128 and its application fields, which can solve the problems of toxic side effects and inability to completely remove the virus

Inactive Publication Date: 2015-10-07
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, since this method cannot completely remove the virus, the patient needs to take medicine for life, but long-term medication has serious side effects

Method used

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  • Polypeptide CJ128 resistant to HIV-1 activity and application of polypeptide CJ128

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, the preparation of polypeptide

[0022] The inventors of the present invention have designed polypeptide CJ128, its amino acid sequence is as follows:

[0023] Polypeptide CJ128 (SEQ ID NO: 1 of the Sequence Listing): MQPIIVAVALVVAIIIAIVRRRRRRRRR.

[0024] Artificially synthesized polypeptide CJ128.

Embodiment 2

[0025] Embodiment 2, the toxicity determination of polypeptide

[0026] 1. Dissolve 1 mg of the polypeptide CJ128 prepared in Example 1 in 20 μl of DMSO, and then perform 2-fold gradient dilution with DMSO to obtain polypeptide dilutions containing different concentrations of the polypeptide.

[0027] 2. Take the white transparent 96-well plate and add the polypeptide dilution prepared in step 1 (1 μl per well).

[0028] 3. Suspend MT-2 cells in RPMI1640 medium containing 10% fetal bovine serum by volume to obtain 1.5×10 5 cells / ml of MT-2 cell suspension.

[0029] 4. Take the MT-2 cell suspension obtained in step 3, add it to the 96-well plate (200 μl per well) that completed step 2, centrifuge briefly, and then 37 ° C, 5% CO 2 Incubate for 72 hours.

[0030] 5. After completing step 4, take the 96-well plate and add 20 μl CellTiter- Luminescent Cell Viability Assay (CellTiter- Luminescence method cell viability detection kit), the luminescence signal was measured by W...

Embodiment 3

[0033] Embodiment 3, the anti-HIV-1 activity determination of polypeptide

[0034]1. Dissolve 1 mg of the polypeptide CJ128 prepared in Example 1 in 20 μl of DMSO, and then perform 2-fold gradient dilution with DMSO to obtain polypeptide dilutions containing different concentrations of the polypeptide.

[0035] 2. Take the white transparent 96-well plate, add the polypeptide dilution prepared in step 1 (1 μl per well)

[0036] 3. Suspend MT-2 cells with RPMI1640 culture medium containing 10% fetal bovine serum by volume percentage to obtain 3.0×10 5 cells / ml of MT-2 cell suspension.

[0037] 4. Adjust the virus concentration in the virus solution of pNL4.3 virus with RPMI1640 culture solution containing 10% fetal bovine serum by volume percentage to obtain a virus dilution solution of 400 TCID50 / ml.

[0038] 5. Mix the MT-2 cell suspension obtained in step 3 with the virus dilution obtained in step 4 in equal volumes, then add the mixed solution to the 96-well plate (200 μl ...

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Abstract

The invention discloses polypeptide CJ128 resistant to HIV-1 activity and application of the polypeptide CJ128. The polypeptide is named as the polypeptide CJ128 and is polypeptide shown in the sequence 1 of a sequence table. The invention further relates to the application of the polypeptide CJ128 in preparing an HIV-resistant drug. The invention further relates to the HIV-resistant drug. The active component of the HIV-resistant drug is the polypeptide CJ128. The polypeptide CJ128 has significant value for treating AIDS.

Description

technical field [0001] The invention relates to a polypeptide CJ128 with anti-HIV-1 activity and its application. Background technique [0002] Human immunodeficiency virus (Human immunodeficiency virus, HIV) is the pathogen that causes human acquired immunodeficiency syndrome (Acquired immunodeficiency syndrome, AIDS). AIDS has spread rapidly around the world since it was first discovered in the United States in 1981. According to the latest UNAIDS report in July 2014, the number of HIV-infected people in the world has reached 35 million. In 2013, there were 63,498 newly diagnosed HIV-infected people in China, and it is estimated that there are currently 780,000 HIV-infected people living in China. [0003] Currently, Highly Active Anti-Retroviral Therapy (HAART), which uses several antiviral drugs in combination, can greatly reduce the viral load in HIV patients and delay the disease process. However, as of now, AIDS is still incurable. With the widespread use of antire...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/16A61K38/16A61P31/18
CPCA61K38/00C07K14/163C12N2740/16022
Inventor 李林李敬云常帅蔡利锋李天一李韩平鲍作义刘永健刘思扬庄道民王晓林
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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