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Applications of bumetanide in inhibition of tumor cell proliferation

A tumor cell proliferation, tumor cell technology, applied in the application field of bumetanide in inhibiting tumor cell proliferation, can solve the problem of renal tubule ineffectiveness, etc.

Active Publication Date: 2016-04-20
BEIJING PROTEOME RES CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Bumetanide is a diuretic that mainly inhibits the active reabsorption of NaCl in the thick-walled segment of the ascending branch of the loop of Henle of the renal tubule, and reabsorbs NaCl in the proximal tubule. + Also inhibits, but has no effect on distal tubules

Method used

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  • Applications of bumetanide in inhibition of tumor cell proliferation
  • Applications of bumetanide in inhibition of tumor cell proliferation
  • Applications of bumetanide in inhibition of tumor cell proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1, the inhibitory effect of bumetanide on A549 cells

[0024] The CCK-8 kit was used to detect the inhibitory effect of bumetanide on A549 cells, and the specific steps were as follows:

[0025] 1. Take a 96-well plate: prepare A549 cells in the logarithmic growth phase into a cell suspension (in the cell suspension, the concentration of A549 cells is 30 cells / microliter) and inoculate them into the test wells and control wells of the 96-well plate , 100 microliters per well; add the complete medium to the blank well of the 96-well plate, 100 microliters per well; then place the 96-well plate at 37°C, 5% CO 2 Cultivate in the incubator for 24h.

[0026] 2. After completing step 1, take the 96-well plate and suck out the culture solution in the well: add different volumes of bumetanide mother solution and complete medium to each well of the test well (so that the volume of the culture system in each well of the test well is equal, Both are 100 microliters, and ...

Embodiment 2

[0029] Example 2, the inhibitory effect of bumetanide on HCT116 cells

[0030]The CCK-8 kit was used to detect the inhibitory effect of bumetanide on HCT116 cells, and the specific steps were as follows:

[0031] 1. Take a 96-well plate: prepare HCT116 cells in the logarithmic growth phase into a cell suspension (in the cell suspension, the concentration of HCT116 cells is 40 cells / microliter) and inoculate them into the test wells and control wells of the 96-well plate , 100 microliters per well; add the complete medium to the blank well of the 96-well plate, 100 microliters per well; then place the 96-well plate at 37°C, 5% CO 2 Cultivate in the incubator for 24h.

[0032] 2. After completing step 1, take the 96-well plate and suck out the culture solution in the well: add different volumes of bumetanide mother solution and complete medium to each well of the test well (so that the volume of the culture system in each well of the test well is equal, Both were 100 microlite...

Embodiment 3

[0035] Example 3, the inhibitory effect of bumetanide on K562 cells

[0036] The CCK-8 kit was used to detect the inhibitory effect of bumetanide on K562 cells, and the specific steps were as follows:

[0037] 1. Take a 96-well plate: prepare K562 cells in the logarithmic growth phase into a cell suspension (in the cell suspension, the concentration of K562 cells is 120 cells / microliter) and inoculate them into the test wells and control wells of the 96-well plate , 50 microliters per well; add complete medium to blank wells of a 96-well plate, 50 microliters per well.

[0038] 2. After completing step 1, take a 96-well plate: add different volumes of bumetanide mother solution and complete medium to each well of the test well (so that the volume of the culture system in each well of the test well is equal, 50 microliters, cloth The final concentrations of Metanide in the culture system were 1, 2, 4, 8, 16, 32, 64, and 128 μg / ml respectively, and each concentration was set up...

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Abstract

The present invention discloses applications of bumetanide in inhibition of tumor cell proliferation. The present invention provides applications of bumetanide in preparation of drug for inhibition of tumor cells, wherein the tumor cells can be lung adenocarcinoma cells, colon cancer cells, leukemia cells, esophageal cancer cells, cervical cancer cells or breast cancer cells, and can specifically be A549 cells, HCT116 cells, K562 cells, Eca109 cells, Hela cells, Jurkat cells or MCF7 cells. According to the present invention, the great value is provided for tumor treatment.

Description

technical field [0001] The invention relates to the application of bumetanide in inhibiting tumor cell proliferation. Background technique [0002] Cancer, also known as malignant tumor (Malignant neoplasm), is a disease caused by the abnormal mechanism of controlling cell growth and proliferation. In addition to growing out of control, cancer cells can also locally invade the surrounding normal tissues and even transfer to other parts of the body through the internal circulatory system or lymphatic system. Common cancers include lung cancer, colon cancer, leukemia, esophageal cancer, cervical cancer, and breast cancer. Lung cancer: Cough is an early symptom of lung cancer. It is characterized by paroxysmal irritating cough. If there is no improvement after 2 weeks of anti-inflammatory treatment, you should be alert to the possibility of lung cancer, or coughing on the basis of the original chronic cough Another warning sign of lung cancer is intermittent repeated small am...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/196A61P35/00A61P35/02
Inventor 姜颖贺福初徐辰孙薇魏汉东
Owner BEIJING PROTEOME RES CENT
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