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Lithium measurement method

A measurement method, lithium reagent technology, applied in the field of lithium measurement, can solve major side effects and other problems

Active Publication Date: 2015-09-30
METALLOGENICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] From this point of view, although lithium salt mood stabilizers have therapeutic effects on patients with depression, bipolar disorder, epilepsy, etc., overdose will cause major side effects. Therefore, when using drugs, the lithium concentration in serum must be constantly monitored at 0.6 Within ~1.2mEq / L, this is a must

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] [Example 1 (lithium reagent combination sample 1)]

[0077] For the lithium reagent composition used in the present invention, the first reagent is prepared as a pH buffer solution, the second reagent is prepared as a color test solution, and the two liquids are mixed immediately before measurement to prepare the lithium reagent composition. It is best to start with these two liquids, as prolonged storage can lead to reagent degradation and should be avoided.

[0078] Here, the preparation method of the reagent composition will be described.

[0079] First, mainly prepare the first reagent as a pH buffer, and its combination is as follows.

[0080] [Example 1 (lithium reagent combination sample 1)]

[0081] (1) The first reagent (as a stabilizer and buffer)

[0082] Chelating agent: no

[0083] Organic solvents: no

[0084] Stabilizer (dispersant: nonionic surfactant):

[0085] TritonX-100 (registered trademark)

[0086] (Octylphenyl polyoxyethylene ether) 1.0% b...

Embodiment 2

[0143] [Example 2 (lithium reagent combination sample 2)]

[0144] Here, Example 2 is described. The combination of Example 2 is essentially the same as Example 1, but Example 2 is not the two-component type of Example 1, but uses a color developing solution to form a one-component type. This is done for the following reasons.

[0145] When dispensing into a 96-well culture plate for a microplate analyzer, in the case of a one-component reagent, only the sample and the chromogenic solution are handled, and in the case of a two-component reagent, it is necessary to separate the sample, The three reagents, the first reagent and the second reagent, are mixed on a small-capacity culture plate. When using a colorimetric tube container for a spectrophotometer with a large capacity of 1mL as in Example 1, the operation is simple, but when using a 96-well culture plate, the operation becomes complicated, so in this Example 2, the A one-component reagent that is easier to handle. Mo...

Embodiment 3

[0183] [Example 3 (lithium reagent combination sample 3)]

[0184] (1) The first reagent (as a stabilizer and buffer)

[0185] Chelating agent: no

[0186] Organic solvents: no

[0187] Stabilizer (dispersant: nonionic surfactant): TritonX-100 (trademark registration)

[0188] (Octylphenyl polyoxyethylene ether) 1.0% by weight

[0189] Masking agent: Triethanolamine 10mM

[0190] As above, add MOPS to 0.1M, adjust to pH 8, add purified water to 1L, and store in a general-purpose storage container.

[0191] (2) The second reagent (as a color test solution)

[0192] Chelating agent: F28 tetraphenylporphyrin 0.5g / L

[0193]Organic solvent: dimethylsulfoxide (DMSO) 20% by weight

[0194] Stabilizer (dispersant: nonionic surfactant): TritonX-100

[0195] (registered trademark) (octylphenyl polyoxyethylene ether) 1.0% by weight

[0196] Masking agent: Triethanolamine 10mM

[0197] MOPS (buffer) was added thereto to make it 0.05 M, adjusted to pH 7.0, purified water was add...

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Abstract

[Problem] To provide to a method for measuring and examining lithium ions in an aqueous solution of a specimen such as biomaterials or environment samples, by using a lithium reagent composition as a coloration reaction reagent and by visual observing or a simple a colorimeter. [Solution] The lithium ions measuring method is characterized by contacting the specimen including serum and plasma test sample with an aqueous solution of a lithium reagent composition comprising a tetraphenyl porphyrin compound, a pH regulator and a pH buffer, by irradiating or expose the resulting solution with white light, and by detecting change in color tone by a visual observation or by detecting the sensitivity by a colorimeter.

Description

technical field [0001] The invention relates to a method for measuring lithium, which is used for measuring lithium in aqueous solutions such as living samples including urine, serum, plasma, and blood test samples, or environmental samples containing drinking water. Background technique [0002] Lithium-containing mood stabilizers have been widely used for the effective treatment of bipolar disorder, epilepsy, and bipolar disorder, but the lithium concentration in serum must be controlled within an appropriate range when taking medication. [0003] Lithium carbonate tablets (administered orally) are usually prescribed as mood stabilizers along with bipolar (manic-depressive) medications or antidepressants. If lithium carbonate (Li 2 CO 3 ) is not close to the blood concentration of lithium poisoning, the drug effect cannot be shown. Since the treatment area and the poisoning area are very similar, it is necessary to specify drug blood concentration monitoring as a necessa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/00G01N31/22
CPCG01N33/15G01N21/25G01N31/22
Inventor 岩渕拓也小田嶋次胜铃木裕子
Owner METALLOGENICS
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