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Continuous efficient cell disruption treatment method based on frequency-mixed ultrasound

A treatment method and frequency mixing technology, applied in the fields of sludge treatment, chemical instruments and methods, water/sludge/sewage treatment, etc., can solve the problems of secondary pollution, ultrasonic power, single frequency, low power, etc., and achieve high efficiency. The effect of treatment and improvement of wall breaking efficiency

Active Publication Date: 2015-09-30
杨浩
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In view of the above situation, the purpose of the present invention is to provide a continuous and high-efficiency cell wall-breaking treatment method based on mixed-frequency ultrasound, which can effectively solve the problems of ultrasonic power, single frequency, low power and secondary pollution in the current ultrasonic biological wall-breaking treatment.

Method used

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  • Continuous efficient cell disruption treatment method based on frequency-mixed ultrasound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 200ml each of the four groups of Microcystis aeruginosa liquid with the same concentration of A, B, C, and D. The ultrasonic output power is 500W. The A, B, and C groups are treated with ultrasound with frequencies of 20KHz, 28KHz and 35KHz, respectively. Irradiation time For 15 seconds, the D group used 20kHz and 28kHz mixing, 28kHz and 35kHz mixing, 20kHz and 35kHz mixing for 5 seconds, the ultrasonically treated algae liquid was placed in the incubator, and the supernatant was taken to measure the absorbance (OD value), compared with the control group without ultrasound, so as to quantify the biological content of cyanobacteria in the solution, as shown in Table 1.

[0021] Table 1

[0022]

[0023] It can be seen from Table 1 that the use of mixed frequency ultrasound to process cyanobacteria can effectively reduce the biological content of cyanobacteria in the solution, thereby increasing the removal rate, and the removal effect of cyanobacteria can be increas...

Embodiment 2

[0025] Take 200ml each of the four groups of Microcystis aeruginosa liquid with the same concentration of A, B, C, and D. The ultrasonic output power is adjusted to 500W. The four groups all use mixed frequency ultrasound. The irradiation time is 15 seconds, 20 seconds, 25 seconds and For 30 seconds, place the ultrasonically treated algae solution in the incubator, take the supernatant to measure the absorbance (OD), and compare it with the control group without ultrasonication to quantify the biological content of the cyanobacteria in the solution, such as Table 2 shows.

[0026] Table 2

[0027]

[0028] OD value

[0029] It can be seen from Table 2 that the mixed-frequency ultrasound is used to treat cyanobacteria. As the action time of mixed-frequency ultrasound increases, the biological content of the cyanobacteria in the solution decreases, and the corresponding removal rate increases. Because this method is designed as a continuous treatment method, The ultrasound a...

Embodiment 3

[0031] Take 200ml each of the four groups of Microcystis aeruginosa liquid with the same concentration of A, B, C and D. The ultrasonic output power is 0.5kW, 1kW, 2kW, 3kW respectively. The mixed ultrasonic action is used for 15 seconds, and the ultrasonically treated algae solution Place it still in the incubator, take the supernatant to measure the absorbance (OD value), and compare it with the control group without ultrasound, so as to quantify the biological content of cyanobacteria in the solution, as shown in Table 3.

[0032] table 3

[0033]

Control group

Group A

Group B

Group C

Group D

Ultrasonic frequency (KHz)

Mixing

Mixing

Mixing

Mixing

Ultrasonic power (kW)

0.5

1

2

3

Action time (Sec)

15

15

15

15

OD value

0.450

0.212

0.171

0.140

0.117

Biological content

47％

38％

31％

26％

[0034] It can be seen from Table 3 that the cyanobacteria are treated with mixed-frequency ultrasound. As the power of the mixed-frequency ultrasound increases, the bio...

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Abstract

The invention provides a continuous efficient cell disruption treatment method based on frequency-mixed ultrasound. According to the method, a sample to be processed is pumped into an ultrasonic reaction vessel, and then frequency-mixed ultrasonic treatment is conducted in the ultrasonic reaction vessel. The ultrasonic reaction vessel is provided with three areas, a set of replaceable power ultrasonic transducers with different frequencies are arranged in each area in a face-to-face mode, power is between 500 W and 3 kW, the frequencies of one set of power ultrasonic transducers are 20 kHz and 28 kHz, the frequencies of another set of power ultrasonic transducers are 28 kHz and 35 kHz, and the frequencies of the other set of power ultrasonic transducers are 20 kHz and 35 kHz. Ultrasonic waves synthesized by two ultrasonic transducers in each set can achieve different frequencies and sound intensities. Different kinds of biological cells in different sizes can be subjected to frequency-mixed ultrasound while passing an ultrasonic treatment area, and then effective disruption treatment is achieved. The method breaks the limitation of the prior art that fixed ultrasonic frequency can only be used for treating specific biological cells and improves disruption efficiency.

Description

Technical field [0001] The invention relates to the technical field of ultrasonic processing, in particular to a continuous high-efficiency cell wall breaking processing method based on mixed-frequency ultrasound. Background technique [0002] With the acceleration of the process of urbanization and industrialization in my country, pollution has become a bottleneck restricting economic development. It is urgent to control environmental pollution and strengthen environmental protection. In many pollution control studies, cell wall breaking has attracted the attention of scientists. For example, the flood of algae in the water body needs to destroy the plant cell wall of the algae, so that the intracellular material can flow out to achieve the purpose of controlling algae pollution; In the process of sludge treatment, because there are a large number of biological cells in the sludge, its combined water in various forms makes the water content of the sludge very large. Therefore, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F1/36C02F11/00
Inventor 杨浩
Owner 杨浩
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