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Laccase mediator ABTS-SiO2 nanoparticles, as well as preparation method and application thereof

A nanoparticle, laccase mediator technology, applied in chemical instruments and methods, immobilized on or in inorganic carriers, water pollutants, etc., can solve the problem of low efficiency, difficult recovery, and increase the cost of laccase mediator use problems, to reduce the cost of use, reduce secondary pollution, and facilitate recycling and reuse.

Inactive Publication Date: 2015-09-23
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the mediators with strong effects are artificially synthesized compounds (such as ABTS). A water-soluble state, if these mediators are used in large quantities, there will be the following problems: one, because the mediators are difficult to recycle after use, and the cost of using the laccase mediator system is increased as the waste liquid is lost; the second , the synthesized mediator has certain toxicity, and discarding it after use will cause environmental pollution; thirdly, how to further recycle the used mediator
Most of the dyes have certain toxicity, are difficult to be absorbed and biodegraded by the environment, affect the survival of aquatic organisms and human health, and cause serious environmental pollution problems. Therefore, a major environmental pollution problem facing today is how to remove dyes in sewage
Finding an efficient and low-cost method for removing dyes from industrial wastewater is a major challenge. The methods that have been applied include adsorption, coagulation, biodegradation, chemical degradation, and photodegradation, but these methods generally have defects such as low efficiency and high cost.

Method used

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  • Laccase mediator ABTS-SiO2 nanoparticles, as well as preparation method and application thereof
  • Laccase mediator ABTS-SiO2 nanoparticles, as well as preparation method and application thereof
  • Laccase mediator ABTS-SiO2 nanoparticles, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Laccase Mediator ABTS-SiO 2 Preparation of nanoparticles

[0017] (1) Weigh 0.548g of ABTS and dissolve it in 10mL of anhydrous methanol, then add 0.224g of potassium tert-butoxide, ultrasonically disperse until uniformly dispersed, stir and react for 2h, filter and wash with anhydrous methanol for 3 times, and then in 45℃ Dry in an oven to remove methanol to obtain off-white 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) dipotassium salt;

[0018] (2) Weigh 0.524g of triphenylphosphine and dissolve it in 20mL of anhydrous acetonitrile, carefully drop about 104μL of bromine in an ice bath until the solution remains light yellow, then add a small amount of triphenylphosphine to make the solution become Colorless, dibromotriphenylphosphine was obtained;

[0019] (3) Add 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) bis(3-ethyl-benzothiazole-6-sulfonic acid) prepared in step (1) to the dibromotriphenylphosphine prepared in step (2) Potassium salt,...

Embodiment 2

[0023] Example 2 ABTS-SiO2 Regenerative use of nanoparticles as laccase mediators

[0024] (1) Take the laccase mediator ABTS-SiO 2 Add 40mg of nanoparticles into 2mL of acetic acid buffer solution with pH=4.5, then add 2.0U of laccase, shake the reaction until complete, until the laccase mediator ABTS-SiO 2 Nanoparticles turn green;

[0025] (2) Add 30 uL of ascorbic acid with a molar concentration of 0.2 mol / L to the mixed liquid after the enzyme-catalyzed reaction in step (1), and stir to react until the laccase mediator ABTS-SiO 2 The nanoparticles changed from green to light yellow, then the reaction mixture was centrifuged, the supernatant was poured off, and the precipitate was washed 5 times with distilled water to recover the obtained laccase mediator ABTS-SiO 2 The nanoparticles are further reused.

Embodiment 3

[0026] Example 3 ABTS-SiO 2 Application of Nanoparticles as Laccase Mediators for Dye Removal

[0027] (1) Take the laccase mediator ABTS-SiO 2 Add 40 mg of nanoparticles to 2 mL of acetic acid buffer solution with pH = 4.5, then add 0.5 U of laccase, then add 0.04 mg of dye indigo carmine, shake the reaction until complete, and the blue solution becomes colorless;

[0028] (2) Add 30 uL of ascorbic acid with a molar concentration of 0.2 mol / L to the mixed solution after the enzyme-catalyzed reaction in step (1), stir and react for 1 min, centrifuge the reaction mixed solution, pour off the supernatant, and then wash the precipitate with distilled water 5 times, the obtained laccase mediator ABTS-SiO was recovered 2 Nanoparticles are used repeatedly as described above. Its effect on the removal of indigo carmine and the changes in repeated use are shown in image 3 , it can be seen from the figure that after 4 times of recycling, it still maintains a high removal effect on...

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Abstract

The invention discloses laccase mediator ABTS-SiO2 nanoparticles, as well as a preparation method and application thereof, and belongs to the technical fields of biology, nano materials and environmental governance. According to the technical scheme, the laccase mediator ABTS-SiO2 nanoparticles are characterized in that ABTS is modified to SiO2 nanoparticles to prepare the laccase mediator ABTS-SiO2 nanoparticles, oxidized ABTS undergoes reduction reaction with a reducing substance to be reduced after the prepared ABTS-SiO2 nanoparticles finishes enzyme-catalyzed reaction as a laccase mediator and the laccase mediator ABTS-SiO2 nanoparticles are centrifugally recovered, so that recycling of the laccase mediator ABTS-SiO2 nanoparticles is realized. The preparation method is simple, low in cost and high in repeatability, and has broad application prospect in the fields of environmental pollution control, chemical synthesis, papermaking, bleaching, energy resources and the like, and the requirement of mass production can be well met.

Description

technical field [0001] The invention belongs to the technical fields of biotechnology, nanometer materials and environmental treatment, and specifically relates to a laccase mediator ABTS-SiO 2 Nanoparticles and methods for their preparation and applications. Background technique [0002] Laccase (EC 1.10.3.2, Diphenol Oxidase) is a multi-copper ion protein belonging to the blue oxidase, which can catalyze a variety of organic substrates including phenols, aromatic amines and inorganic compounds. electron acceptor, the only by-product produced is water. Laccases are a very important class of enzymes due to their broad substrate scope and their applications in many industries and biotechnology. Research reports have shown that when laccase is used alone, it cannot fully catalyze substrates with high redox potential. However, after adding some substances called laccase mediators, such as 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS), etc., can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C02F3/34C02F101/34
Inventor 黄娟刘友勋周鑫平付云李翠萍闫明阳李长正
Owner XINXIANG MEDICAL UNIV
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